Functional analysis of Salmonella type III effectors, which are involved in evading the host innate immune response.

• Project/Area Number: 24590534
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Kitasato University
• Principal Investigator: HANEDA Takeshi 北里大学, 薬学部, 講師 (00348591)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: サルモネラ / III型エフェクター / 自然免疫 / 自然免疫回避 / NF-κB不活化 / 炎症制御

Outline of Final Research Achievements

In this study, we demonstrated that Salmonella anti-inflammatory type III effector SpvC, a phosphothreonine lyase, contributes to bacterial proliferation in the spleen in the mouse model of systemic infection. We also revealed that other Salmonella type III effectors, SseK1, SseK2 and SseK3, regulate NF-κB activation in the host cell.

Research Products

• [Journal Article] Functional Characterization of the Type III Secretion ATPase SsaN Encoded by Salmonella Pathogenicity Island 2 (2014)
  • Author(s): Yukie Yoshida, Tsuyoshi Miki, Sayaka Ono, Takeshi Haneda, Masahiro Ito, Nobuhiko Okada
  • Journal Title: PLOS ONE Volume: 9 Issue: 4 Pages: e94347-e94347
  • DOI: 10.1371/journal.pone.0094347
  • Peer Reviewed / Open Access
• [Presentation] The functional analysis of Salmonella type III effector, SpvC (2013)
  • Author(s): Takeshi Haneda, Nobuhiko Okada
  • Organizer: 4th ASM Conference on Salmonella
  • Place of Presentation: Seaport Hotel and World Trade Center (Boston, MA, USA)
• [Presentation] サルモネラIII型エフェクターによる炎症制御機構の解析
  • Author(s): 羽田健、岡田信彦
  • Organizer: 第15回北里微生物アカデミー
  • Place of Presentation: 北里大学相模原キャンパス(相模原市)
• [Presentation] サルモネラの自然免疫回避エフェクターの機能解析
  • Author(s): 羽田健、岡田信彦
  • Organizer: 第31回白金シンポジウム
  • Place of Presentation: 北里大学白金キャンパス(東京都港区)
• [Remarks] 北里大学薬学部微生物学教室
  • URL: http://www.pharm.kitasato-u.ac.jp/microbiology/