Genome and virulence factor analysis of streptococci affected to chronic inflammatory diseases including primary biliary cirrhosis

• Project/Area Number: 24590536
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Tokyo Women's Medical University (2014-2015); Juntendo University (2012-2013)
• Principal Investigator: KIKUCHI KEN 東京女子医科大学, 医学部, 教授 (60214748)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 原発性胆汁性肝硬変 / S. intermedius / histone-like protein / S. anginosus group / competence / HLP / S. anginosus / S. constellatus / transformation / gp210 / molecular mimic / ELISA / horizontal transfer / house keeping gene / Streptococcus anginosus

Outline of Final Research Achievements

Chronic inflammation like periodontitis caused by Streptococcus intermedius (SI) contributed to the etiology and progression of primary biliary cirrhosis (PBC), which occurred in menopausal females mainly. The continuous and localized production of histone-like protein was one of the mechanism of the pathogenesis of PBC. SI, one of the members of S. anginosus group, had uniform genetic backgrounds compared as other species, and the result was due to co-evolution with human in specialized niche environments.

Research Products

• [Journal Article] A Problem of Species Determination Using Housekeeping Genes in the Streptococcus anginosus Group (2015)
  • Author(s): Mizutani T, Iguchi S, Hiramatsu K, Kikuchi K
  • Journal Title: Juntendo Medical Journal Volume: 61 Pages: 608-616
  • NAID: 130005133295
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 口腔細菌と自己免疫疾患 (2015)
  • Author(s): 菊池 賢
  • Journal Title: Bioindustry Volume: 32 Pages: 20-24
• [Journal Article] 感染症四方山話（10）:感染症研究との出会い (2015)
  • Author(s): 菊池 賢
  • Journal Title: The Chemical Times Volume: 237 Pages: 2-7
  • Open Access
• [Journal Article] Catheter-related blood stream infection caused by Dermacoccus barathri, representing the first case of Dermacoccus infection in humans. (2015)
  • Author(s): Takahashi N, Shinjoh M, Tomita H, Fujino A, Sugita K, Katohno Y, Kuroda T, Kikuchi K
  • Journal Title: J Infect Chemother Volume: 21 Issue: 8 Pages: 613-616
  • DOI: 10.1016/j.jiac.2015.04.007
  • Peer Reviewed / Open Access
• [Journal Article] 口腔細菌と自己免疫疾患 (2015)
  • Author(s): 菊池 賢
  • Journal Title: BIOINDUSTRY Volume: 32 Pages: 20-24
• [Journal Article] Development of an appropriate PCR system for the reclassification of Streptococcus suis. (2014)
  • Author(s): Ishida S, Tien LHT, Osawa R, Tohya M, Nomoto R, Kawamura Y, Takahashi T, Kikuchi N, Kikuchi K, Sekizaki T.
  • Journal Title: J Microbiol Meth Volume: 107 Pages: 66-70
  • DOI: 10.1016/j.mimet.2014.09.003
  • Peer Reviewed
• [Journal Article] Frequent occurrence of fever in patients who have undergone endoscopic submucosal dissection for colorectal tumor, but bacteremia is not a significant cause. (2014)
  • Author(s): Izumi K, Osada T, Sakamoto N, Kodani T, Higashihara Y, Ritsumo H, Shibuya T, Nagahara A, Ogihara T, Kikuchi K, Watanabe S.
  • Journal Title: Surg Endosc Volume: 28 Issue: 10 Pages: 2899-2904
  • DOI: 10.1007/s00464-014-3551-5
  • Peer Reviewed
• [Journal Article] A streptolysin S homologue is essential for β-haemolytic Streptococcus constellatus subsp. constellatus cytotoxicity (2014)
  • Author(s): Atsushi Tabata, Yuji Sato, Kentaro Maya, Kota Nakano, Ken Kikuchi, Robert Whiley, Kazuto Ohkura, Toshifumi Tomoyasu, Hideaki Nagamune
  • Journal Title: Microbiology Volume: 160 Issue: 5 Pages: 980-991
  • DOI: 10.1099/mic.0.075580-0
  • Peer Reviewed
• [Journal Article] A case of prosthetic valve endocarditis caused by Streptococcus constellatus, as a rare agent of endocarditis. (2013)
  • Author(s): Ko T, Mahara K, Ota M, Kato Y, Takanashi S, Kikuchi K, Umemura J, Sumiyoshi T, Tomoike H.
  • Journal Title: Heart Lung Volume: 42 Issue: 5 Pages: 379-381
  • DOI: 10.1016/j.hrtlng.2013.05.006
  • Peer Reviewed
• [Journal Article] LacR mutations are frequently observed in Streptococcus intermedius and are responsible for increased intermedilysin production and virulence. (2013)
  • Author(s): Tomoyasu T, Imaki H, Masuda S, Okamoto A, Kim HJ, Waite RD, Whiley RA, Kikuchi K, Hiramatsu K, Tabata A and Nagamune H.
  • Journal Title: Infect. Immun. Volume: 81 Issue: 9 Pages: 3276-3286
  • DOI: 10.1128/iai.00638-13
  • Peer Reviewed
• [Journal Article] Novel twin streptolysin S-like peptides encoded in the sag operon homologue of β-hemolytic Streptococcus anginosus (2013)
  • Author(s): Atsushi Tabata, Kota Nakano, Kazuto Ohkura, Toshifumi Tomoyasu, Ken Kikuchi, Robert Whiley, Hideaki Nagamune
  • Journal Title: Journal of Bacteriology Volume: 195 Issue: 5 Pages: 1090-1099
  • DOI: 10.1128/jb.01344-12
  • Peer Reviewed
• [Journal Article] 菌血症・敗血症―その2 (2013)
  • Author(s): 菊池 賢
  • Journal Title: The Chemical Times Volume: 228 Pages: 24-27
• [Journal Article] Overview of new approaches in bacterial identification and characterization (2013)
  • Author(s): Kikuchi K
  • Journal Title: J Infect Chemother Volume: 19
  • Peer Reviewed
• [Journal Article] Detection of IMP metallo-β-lactamase in carbapenem-nonsusceptible Enterobacteriaceae and glucose non-fermenting Gram-negative rods by immunochromatography assay. (2013)
  • Author(s): Notake S, Matsuda M, Tamai K, Yanagisawa H, Hiramatsu K, Kikuchi K*.
  • Journal Title: J. Clin. Microbiol. Volume: 51
  • Peer Reviewed
• [Journal Article] Involvement of commensal bacteria may lead to dysregulated inflammatory and autoimmune responses in a mouse model for chronic nonsuppurative destructive cholangitis (2012)
  • Author(s): HARUTA Ikuko, KIKUCHI Ken, NAKAMURA Minoru, HIROTA Katsuhiko, KATO Hidehito, MIYAKAWA Hiroshi, SHIBATA Noriyuki, MIYAKE Yoichiro, HASHIMOTO Etsuko, SHIRATORI Keiko, YAGI Junji
  • Journal Title: Journal of clinical immunology Volume: 32(5) Issue: 5 Pages: 1026-1037
  • DOI: 10.1007/s10875-012-9712-1
  • Peer Reviewed
• [Journal Article] Evaluation of a simple protein-extraction method for species identification of clinically relevant staphylococci by matrix-assisted laser desorption ionization-time of flight mass spectrometry. (2012)
  • Author(s): Matsuda N, Matsuda M, Notake S, Yokokawa H, Kawamura Y, Hiramatsu K, Kikuchi K*.
  • Journal Title: J. Clin. Microbiol. Volume: 50 Issue: 12 Pages: 3862-3866
  • DOI: 10.1128/jcm.01512-12
  • Peer Reviewed
• [Journal Article] 感染性心内膜炎 (2012)
  • Author(s): 菊池 賢
  • Journal Title: The Chemical Times Volume: 226 Pages: 8-13
  • NAID: 10021192988
• [Journal Article] 菌血症・敗血症―その1 (2012)
  • Author(s): 菊池 賢
  • Journal Title: The Chemical Times Volume: 227 Pages: 19-23
• [Presentation] Streptococcus anginosus groupのcompetence factor: comCDEの多様性とhouse keeping geneのhorizontal transfer (2014)
  • Author(s): 菊池 賢、井口成一、水谷友紀、平松啓一
  • Organizer: 第46回レンサ球菌研究会
  • Place of Presentation: 東京大学農学部中島菫一郎記念ホール（東京都・文京区）
  • Year and Date: 2014-06-27 – 2014-06-28
• [Presentation] β溶血性アンギノーサス群レンサ球菌が保有するストレプトリジンSホモログの多様性 (2014)
  • Author(s): 田端厚之、眞屋健太朗、大谷浩美、佐藤裕士、中野晃太、大倉一人、菊池 賢、友安俊文、長宗秀明
  • Organizer: 第46回レンサ球菌研究会
  • Place of Presentation: 東京大学農学部中島菫一郎記念ホール（東京都・文京区）
  • Year and Date: 2014-06-27 – 2014-06-28
• [Presentation] Streptococcus anginosus group (SAG) のcompetence factor：ComCDEの多様性 (2014)
  • Author(s): 菊池 賢、井口成一、水谷友紀、平松啓一
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京
• [Presentation] Streptococcus anginosus groupにおけるhouse keeping geneのhorizontal transfer (2013)
  • Author(s): 菊池 賢
  • Organizer: 第22回Lancefieldレンサ球菌研究会
  • Place of Presentation: 東京
• [Presentation] 臨床微生物領域におけるMALDI-TOF MSの応用と限界
  • Author(s): 菊池 賢
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京
  • Invited
• [Presentation] Streptococcus anginosus group (SAG) のcompetence factor: ComCDEの多様性 とhouse keeping geneのhorizontal gene transfer
  • Author(s): 菊池 賢、松田真理、井口成一、水谷友紀、平松啓一
  • Organizer: 第46回レンサ球菌研究会
  • Place of Presentation: 東京
• [Presentation] 原発性胆汁性肝硬変、自己免疫性膵炎、および類縁患者 歯周病病変部におけるS. anginosusu group解析の分布と分類
  • Author(s): 菊池 賢、春田郁子、清水京子、橋本悦子、白鳥敬子、岡本俊宏、 安藤智博、八木淳二、水谷友紀、井口成一、平松啓一
  • Organizer: 第22回Lancefieldレンサ球菌研究会・第45回レンサ球菌感染症研究会
  • Place of Presentation: 東京
• [Presentation] S. anginosus groupにおける遺伝子同定上の問題
  • Author(s): 水谷友紀、菊池 賢、平松啓一
  • Organizer: 第22回Lancefieldレンサ球菌研究会・第45回レンサ球菌感染症研究会
  • Place of Presentation: 東京