| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Enterohemorrhagic Escherichia coli (EHEC) produces Stx1 and Stx2 causing severe diseases like hemolytic uremic syndrome (HUS). As there is no vaccine or no curative for these diseases caused by both toxins, we have been trying to isolate monoclonal antibody or polyclonal antibodies cross-reactively neutralizing both toxins. We already reported that nasal subunit-vaccine such as Stx2B-His with a mutant heat-labile enterotoxin induced polyclonal antibodies cross-reactively neutralizing both toxins （Vaccine,24:3591,2006、26:469,2008、26:2092.2008）.Then, in this project, we try to isolate a monoclonal antibody, which will neutralize both toxins cross-reactively and use for the treatment of severe diseases like HUS caused by both toxins.
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