Analysis of function of IEE, a novel enhancer for IS excision in O157

• Project/Area Number: 24590543
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: KUSUMOTO Masahiro 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所 細菌・寄生虫研究領域, 主任研究員 (40548210)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 腸管出血性大腸菌 / O157 / IEE / integrative element / 切り出し / プロファージ様エレメント / DNAマイクロアレイ

Outline of Final Research Achievements

Insertion sequence (IS)-excision enhancer (IEE) promotes excision of IS elements and generates various genomic deletions that lead to the diversification of the bacterial genome in enterohemorrhagic Escherichia coli (EHEC). In this study, we show that IEE can be transferred via SpLE1 integrative element and promotes not only IS excision but also excision of the SpLE1. Our data suggested that IEE may be a multifunctional protein that promotes excision of IS elements or SpLE1 in cooperation with IS transposase or SpLE1 integrase, respectively.

Research Products

• [Journal Article] Lineage-specific distribution of insertion sequence excision enhancer in enterotoxigenic Escherichia coli isolated from swine (2014)
  • Author(s): Masahiro Kusumoto, Dai Fukamizu, Yoshitoshi Ogura, Eiji Yoshida, Fumiko Yamamoto, Taketoshi Iwata, Tadasuke Ooka, Masato Akiba, Tetsuya Hayashi
  • Journal Title: Applied and Environmental Microbiology Volume: 80 Issue: 4 Pages: 1394-1402
  • DOI: 10.1128/aem.03696-13
  • Peer Reviewed
• [Presentation] 豚由来病原性大腸菌の進化系統と保有遺伝子の多様性 (2015)
  • Author(s): 楠本正博
  • Organizer: 平成26年度日本獣医師会獣医学術学会年次大会
  • Place of Presentation: 岡山コンベンションセンター（岡山市）
  • Year and Date: 2015-02-13 – 2015-02-15
  • Invited
• [Presentation] ブタから分離された毒素原性大腸菌が保有する腸管出血性大腸菌プロファージ様エレメントの解析 (2014)
  • Author(s): 楠本正博, 深水大, 小椋義俊, 吉田英二, 山本史子, 岩田剛敏, 大岡唯祐, 秋庭正人, 林哲也
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（札幌市）
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] IEEはプロファージ様エレメントSpLE1の転移にも関与する (2014)
  • Author(s): 楠本正博, 小椋義俊, 大岡唯祐, 李謙一, 岩田剛敏, 秋庭正人, 林哲也
  • Organizer: 第18回腸管出血性大腸菌感染症研究会
  • Place of Presentation: 同志社大学（京都市）
  • Year and Date: 2014-07-15 – 2014-07-16