Comparative genome analysis of M. leprae

• Project/Area Number: 24590544
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: KAI MASANORI 国立感染症研究所, その他部局等, 室長 (10291147)
• Co-Investigator(Renkei-kenkyūsha): KURODA Makoto 国立感染症研究所, 病原体ゲノム解析研究センター, センター長 (80317411) ; SEKIZUKA Takeshi 国立感染症研究所, 病原体ゲノム解析研究センター, 主任研究官 (40462775)
• Research Collaborator: NAKATA Noboru
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ハンセン病 / らい菌 / ゲノム / 少菌型 / 多菌型 / SNPs / genotyping

Outline of Final Research Achievements

Leprosy is caused by Mycobacterium leprae and classified into multi-bacillary (MB) and pauci-bacillary (PB). Objectives and results of this study were as follows. (1) The genome sequence of M. leprae isolated from MB patient in Honshu islands of Japan was determined and compared with two genome sequences (isolated from India and Brazil) registered in database. Some characteristic features were found in the genome of Japanese isolate. (2) The SNPs pattern on the regions in M. leprae strains isolated in East Asia or Southeast Asia was investigated. And we found that many of Korean isolates and Honshu isolates showed identical characteristic SNPs pattern. (3) The number of M. leprae bacilli in PB patient was extremely small. We acquired some information of genome sequence in M. leprae derived from PB patient but not finished completely.

Research Products

• [Journal Article] Hp-rPCR法を用いたらい菌薬剤耐性変異の検出 (2014)
  • Author(s): 甲斐雅規
  • Journal Title: 日本ハンセン病学会雑誌 Volume: 83 Pages: 6-13
  • NAID: 130005169033
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Genome-wide re-sequencing of multidrug-resistant Mycobacterium leprae Airaku-3 (2014)
  • Author(s): Singh P, Benjak A, Carat S, Kai M, Busso P, Avanzi C, Paniz-Mondolfi A, Peter C, Harshman K, Rougemont J, Matsuoka M, Cole ST
  • Journal Title: Clin Microbiol Infect Volume: 20 Issue: 10 Pages: 0619-0622
  • DOI: 10.1111/1469-0691.12609
  • Peer Reviewed / Open Access
• [Journal Article] Complete genome sequence and comparative genomic analysis of Mycobacterium massiliense JCM 15300 in the Mycobacterium abscessus group reveal a conserved genomic island MmGI-1 related to putative lipid metabolism (2014)
  • Author(s): Sekizuka T, Kai M, Nakanaga K, Nakata N, Kazumi Y, Maeda S, Makino M, Hoshino Y, Kuroda M
  • Journal Title: PLoS One Volume: 9 Issue: 12 Pages: e114848-e114848
  • DOI: 10.1371/journal.pone.0114848
  • Peer Reviewed / Open Access
• [Journal Article] Chaacteristic mutations found in the ML0411 gene of Mycobacterium leprae isolated in Northeast Asian countries (2013)
  • Author(s): M. Kai, N. Nakata, M. Matsuoka, T. Sekizuka, M. Kuroda, M. Makino
  • Journal Title: Infection, Genetics and Evolution Volume: 19 Pages: 200-204
  • DOI: 10.1016/j.meegid.2013.07.014
  • Peer Reviewed
• [Presentation] Analysis of M. leprae strains from Japan: new trends in phylogeography (2015)
  • Author(s): Avanzi C, Benjak A, Singh P, Busso P, Matsuoka M, Kai M, Cole S
  • Organizer: 25th European Society of Clinical Microbiology and Infectious Diseases
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2015-04-25 – 2015-04-28
• [Presentation] 日本で分離されたらい菌Kyoto-2の特徴的なゲノタイプ (2015)
  • Author(s): 甲斐雅規、中田 登、関塚剛史、黒田 誠、牧野正彦
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 長良川国際会議場
  • Year and Date: 2015-03-26 – 2015-03-27
• [Presentation] らい菌Kyoto-2株の増殖能への関与が疑われる遺伝子の解析 (2014)
  • Author(s): 甲斐雅規、中田 登、松岡正典、牧野正彦
  • Organizer: 第87回日本ハンセン病学会総会・学術大会
  • Place of Presentation: 所沢市民文化センター
  • Year and Date: 2014-09-29 – 2014-09-30
• [Presentation] Comparison of genome sequences between Mycobacterium leprae prepared before and after passaging in nude mice footpad (2014)
  • Author(s): M. Kai, T. Sekizuka, A. A. Maghanoy, M. F. Balagon, P. Saunderson, M. Makino, M. Kuroda
  • Organizer: XVIII International Symposium on Gnotobiology
  • Place of Presentation: St-Petersburg, Russian Federation
  • Year and Date: 2014-09-21 – 2014-09-24
• [Presentation] Characteristic SNPs in Mycobacterium leprae isolated in Japan (2013)
  • Author(s): M. Kai, N. Nakata, G.T. Chae, P. Saunderson, A.A. Maghanoy. M.F. Balagon, M. Matsuoka, T. Sekizuka, M. Kuroda, M. Makino
  • Organizer: 18th International Leprosy Congress
  • Place of Presentation: Brussels, Belgium
• [Presentation] Characteristic SNPs in Mycobacterium leprae isolated in Japan (2013)
  • Author(s): 甲斐雅規
  • Organizer: 第18回国際ハンセン病学会
  • Place of Presentation: Management Center Europe (Belgium)
• [Presentation] らい菌Kyoto-2株の全ゲノムシーケンスにより同定されたSNPsの解析 (2012)
  • Author(s): 甲斐雅規
  • Organizer: 日本ハンセン病学会
  • Place of Presentation: 北海道大学学術交流会館（北海道）