Clarification of the mechanistic analysis of the samll RNA derived from HBV geneome and its asociation for hepatocarcinogenesis

• Project/Area Number: 24590559
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: Nippon Medical School
• Principal Investigator: MIZUGUCHI Yoshiaki 日本医科大学, 医学部, 助教 (70409217)
• Co-Investigator(Renkei-kenkyūsha): TAKIZAWA Toshihiro 日本医科大学, 大学院医学研究科, 教授 (90271220) ; DEMETRIS Anthony J University of Pittsburgh Pathology, Professor
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: microRNA / HBV

Outline of Final Research Achievements

We constructed a plasmid vector that was expressing 26nt RNA that might be derived from HBV genome, and we transfected the vector in to the HCC cell line HepB3 cells.Unfortunately, we did not detected the expression of the 26nt small RNA in the cell line.
We also constructed stably expressing cell lines. Some of them did EMT by the transfection, which indicated that the small RNA had a potential for initiate EMT by undetermined mechanism.

Research Products

• [Journal Article] Small proline rich protein 2a in benign and malignant liver disease (2014)
  • Author(s): Yoshiaki mizuguchi
  • Journal Title: Hepatology Volume: 53 Issue: 3 Pages: 1130-53
  • DOI: 10.1002/hep.26889
  • Peer Reviewed
• [Journal Article] Mir-376c down-regulation accelerates EGF-dependent migration by targeting GRB2 in the HuCCT1 human intrahepatic cholangiocarcinoma cell line. (2013)
  • Author(s): Iwaki J, Kikuchi K, Mizuguchi Y, Kawahigashi Y, Yoshida H, Uchida E, Takizawa T.
  • Journal Title: PLoS ONE Volume: 8 Issue: 7 Pages: e69496-e69496
  • DOI: 10.1371/journal.pone.0069496
  • Peer Reviewed
• [Journal Article] Cooperation of p300 and PCAF in the control of microRNA 200c/141 transcription and epithelial characteristics (2012)
  • Author(s): Mizuguchi Y, Specht S, Lunz JG 3rd, Isse K, Corbitt N, Takizawa T, Demetris AJ.
  • Journal Title: Plos one Volume: 7 Issue: 2 Pages: e32449-e32449
  • DOI: 10.1371/journal.pone.0032449
  • Peer Reviewed
• [Journal Article] Gene Suppression of Mouse Testis In Vivo Using Small Interfering RNA Derived from Plasmid Vectors (2012)
  • Author(s): Takizawa Ta, Ishikawa T, Kosuge T, Mizuguchi Y, Sato Y, Koji T, Araki Y, Takizawa To.
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 45 Issue: 1 Pages: 77-81
  • DOI: 10.1267/ahc.11024
  • NAID: 130001854310
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed
• [Journal Article] SPRR2A enhances p53 deacetylation through HDAC1 and down regulates p21 promoter activity (2012)
  • Author(s): Mizuguchi Y, Specht S, Lunz JG 3rd, Isse K, Corbitt N, Takizawa T, Demetris AJ.
  • Journal Title: BMC Mol Biol Volume: 13:20 Issue: 1
  • DOI: 10.1186/1471-2199-13-20
  • Peer Reviewed
• [Journal Article] SnoN/SKIL modulates proliferationthrough control of hsa-miR-720 transcription in esophageal cancer cells. (2011)
  • Author(s): Eriko Shinozuka, Masao Miyashita, Yoshiaki Mizuguchi, Ichiro Akagi, Kunio Kikuchi, Hiroshi Makino, Takeshi Matsutani, Nobutoshi Hagiwara, Tsutomu Nomura, Eiji Uchida, Toshihiro Takizawa.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 430(1) Issue: 1 Pages: 101-106
  • DOI: 10.1016/j.bbrc.2012.11.005
  • Peer Reviewed
• [Book] Bioinfromatics (2012)
  • Author(s): Yoshiaki Mizuguchi, Takuya Mishima, Eiji Uchida, Toshihiro Takizawa
  • Total Pages: 11
  • Publisher: Intech