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Development and application of rotavirus reverse genetics system

Research Project

Project/Area Number 24590561
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Virology
Research InstitutionFujita Health University

Principal Investigator

KOMOTO Satoshi  藤田保健衛生大学, 医学部, 講師 (60367711)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsロタウイルス / リバースジェネティクス / ヘルパーウイルス / リバースジェネティクス系 / レオウイルス / T7 RNAポリメラーゼ / VP4
Outline of Final Research Achievements

Rotavirus VP4 possesses three conserved mono-basic residues at its trypsin cleavage site (R231, R241, and R247). Although only R247 is assumed to be required for the activation of infectivity with conventional techniques, the strict conservation of the three residues suggests that the presence of these residues may play an important role during infection. This possibility was evaluated by generating recombinant rotaviruses with trypsin cleavage site mutations using reverse genetics.
We isolated and characterized a rotavirus variant possessing a rearranged segment 11 with small sequence (3-bp)deletion within the open reading frame. Our results indicate that a rotavirus genomic rearrangement impairs packaging efficiency into the progeny viruses despite does not confer growth disadvantage to viruses.
We developed a plasmid-based reverse genetics system for reovirus driven by a plasmid-encoded T7 RNA polymerase, which could increase the flexibility of such reverse genetics systems.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (14 results)

All 2015 2014 2013 2012

All Journal Article (9 results) (of which Peer Reviewed: 8 results,  Open Access: 1 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Whole genomic analysis of human G12P[6] and G12P[8] rotavirus strains that have emerged in Myanmar.2015

    • Author(s)
      Ide T, Komoto S, Higo-Moriguchi K, Htun KW, Myint YY, Myat TW, Thant KZ, Thu HM, Win MM, Oo HN, Htut T, Wakuda M, Dennis FE, Haga K, Fujii Y, Katayama K, Rahman S, Nguyen SV, Umeda K, Oguma K, Tsuji T, Taniguchi K.
    • Journal Title

      PLoS ONE

      Volume: 10 Issue: 5 Pages: 1-24

    • DOI

      10.1371/journal.pone.0124965

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ロタウイルス2014

    • Author(s)
      河本聡志、谷口孝喜
    • Journal Title

      日本ウイルス学会誌

      Volume: 64 Pages: 179-190

    • NAID

      130005103364

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Whole genomic analysis of porcine G10P[5] rotavirus strain P343 provides evidence for bovine-to-porcine interspecies transmission.2014

    • Author(s)
      Komoto S, Pongsuwanna Y, Ide T, Wakuda M, Guntapong R, Dennis FE, Haga K, Fujii Y, Katayama K, Taniguchi K.
    • Journal Title

      Vet Microbiol

      Volume: 174 Issue: 3-4 Pages: 577-583

    • DOI

      10.1016/j.vetmic.2014.09.033

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Whole genomic analysis of human G12P[6] and G12P[8] rotavirus strains that have emerged in Kenya: Identification of porcine-like NSP4 genes.2014

    • Author(s)
      Komoto S, Wandera Apondi E, Shah M, Odoyo E, Nyangao J, Tomita M, Wakuda M, Maeno Y, Shirato H, Tsuji T, Ichinose Y, Taniguchi K.
    • Journal Title

      Infect Genet Evol

      Volume: 27 Pages: 277-293

    • DOI

      10.1016/j.meegid.2014.08.002

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A plasmid-based reverse genetics system for mammalian orthoreoviruses driven by a plasmid-encoded T7 RNA polymerase2014

    • Author(s)
      Satoshi Komoto
    • Journal Title

      Journal of Virological Methods

      Volume: 196 Pages: 36-39

    • DOI

      10.1016/j.jviromet.2013.10.023

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Case of Mild Encephalopathy with a Reversible Splenial Lesion Associated with G5P[6]Rotavirus Infection.2013

    • Author(s)
      Matsuoka T, Yodoshi T, Sugai M, Hiyane M, Matsuoka T, Akeda H, Ohfu M, Komoto S, Taniguchi K.
    • Journal Title

      Case Rep Pediatr

      Volume: 2013 Pages: 197163-197163

    • DOI

      10.1155/2013/197163

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genetic engineering of rotaviruses by reverse genetics.2013

    • Author(s)
      Komoto S, Taniguchi K.
    • Journal Title

      Microbiol Immunol

      Volume: 57 Issue: 7 Pages: 479-486

    • DOI

      10.1111/1348-0421.12071

    • NAID

      40019719032

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Whole genomic analysis of a porcine-like human G5P[6] rotavirus strain isolated from a child with diarrhoea and encephalopathy in Japan.2013

    • Author(s)
      Komoto S, Maeno Y, Tomita M, Matsuoka T, Ohfu M, Yodoshi T, Akeda H, Taniguchi K.
    • Journal Title

      J Gen Virol

      Volume: 94 Issue: 7 Pages: 1568-1575

    • DOI

      10.1099/vir.0.051011-0

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genetics and reverse genetics of rotavirus.2012

    • Author(s)
      Taniguchi K, Komoto S.
    • Journal Title

      Curr Opin Virol

      Volume: 2 Issue: 4 Pages: 399-407

    • DOI

      10.1016/j.coviro.2012.06.001

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] タイで検出されたG10P[5]ブタロタウイルスP343株ゲノムの全塩基配列解析2014

    • Author(s)
      河本聡志、井手富彦、和久田光毅、Dennis Francis Ekow、芳賀慧、藤井克樹、片山和彦、谷口孝喜
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜 会議センター
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] ミャンマーにおけるG12P[6]およびG12P[8]ヒトロタウイルスの全塩基配列に基づく遺伝子解析2014

    • Author(s)
      井手富彦、河本聡志、守口匡子、Dennis Francis Ekow、芳賀慧、藤井克樹、片山和彦、Shofiqur Rahman、梅田浩二、Sa Van Nguyen、辻孝雄、谷口孝喜
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜 会議センター
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] T7 RNAポリメラーゼ発現プラスミドを用いたレオウイルスの遺伝子操作系2013

    • Author(s)
      河本聡志、川岸崇裕、冨田万祐子、小林剛、谷口孝喜
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] ロタウイルス遺伝子操作系の開発とそれを用いた外殻スパイク蛋白質VP4の解析2012

    • Author(s)
      河本聡志
    • Organizer
      第60回日本ウイルス学会学術集会
    • Place of Presentation
      グランキューブ大阪(大阪国際会議場)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] ロタウイルスのVP4トリプシン開裂部位の解析2012

    • Author(s)
      河本聡志、冨田万祐子、谷口孝喜
    • Organizer
      第60回日本ウイルス学会学術集会
    • Place of Presentation
      グランキューブ大阪(大阪国際会議場)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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