Development and application of rotavirus reverse genetics system
Project/Area Number |
24590561
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Virology
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Research Institution | Fujita Health University |
Principal Investigator |
KOMOTO Satoshi 藤田保健衛生大学, 医学部, 講師 (60367711)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | ロタウイルス / リバースジェネティクス / ヘルパーウイルス / リバースジェネティクス系 / レオウイルス / T7 RNAポリメラーゼ / VP4 |
Outline of Final Research Achievements |
Rotavirus VP4 possesses three conserved mono-basic residues at its trypsin cleavage site (R231, R241, and R247). Although only R247 is assumed to be required for the activation of infectivity with conventional techniques, the strict conservation of the three residues suggests that the presence of these residues may play an important role during infection. This possibility was evaluated by generating recombinant rotaviruses with trypsin cleavage site mutations using reverse genetics. We isolated and characterized a rotavirus variant possessing a rearranged segment 11 with small sequence (3-bp)deletion within the open reading frame. Our results indicate that a rotavirus genomic rearrangement impairs packaging efficiency into the progeny viruses despite does not confer growth disadvantage to viruses. We developed a plasmid-based reverse genetics system for reovirus driven by a plasmid-encoded T7 RNA polymerase, which could increase the flexibility of such reverse genetics systems.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Whole genomic analysis of human G12P[6] and G12P[8] rotavirus strains that have emerged in Myanmar.2015
Author(s)
Ide T, Komoto S, Higo-Moriguchi K, Htun KW, Myint YY, Myat TW, Thant KZ, Thu HM, Win MM, Oo HN, Htut T, Wakuda M, Dennis FE, Haga K, Fujii Y, Katayama K, Rahman S, Nguyen SV, Umeda K, Oguma K, Tsuji T, Taniguchi K.
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Journal Title
PLoS ONE
Volume: 10
Issue: 5
Pages: 1-24
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Whole genomic analysis of human G12P[6] and G12P[8] rotavirus strains that have emerged in Kenya: Identification of porcine-like NSP4 genes.2014
Author(s)
Komoto S, Wandera Apondi E, Shah M, Odoyo E, Nyangao J, Tomita M, Wakuda M, Maeno Y, Shirato H, Tsuji T, Ichinose Y, Taniguchi K.
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Journal Title
Infect Genet Evol
Volume: 27
Pages: 277-293
DOI
Related Report
Peer Reviewed
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