| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Outline of Final Research Achievements |
Tumorigenesity of CD25-negative and -positive CD4 T cells isolated from HTLV-1-infected humanized mice that exhibit ATL-like symptoms was examined by transplanting cells to the spleen of NOG mouse and only CD25-positive HTLV-1-infected CD4 T cells was found to form tumor. cDNA microarray analysis demonstrated that several genes related to the regulatory T-cells and a cellular mutator gene, APOBEC3B, were activated upon HTLV-1 infection. Genes for BATF3 and SYK were upregulated in parallel with the expression of CD25. Since no sign of mutation in the level of host genome was detected, the epigenetic modifications are suggested to be mainly involved in the leukemogenesis of HTLV-1 infected humanized mouse.
|
