Study on molecular and structural basis of anti-retroviral activity by APOBEC3
Project/Area Number |
24590568
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Virology
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Research Institution | National Hospital Organization Nagoya Medical Center |
Principal Investigator |
IWATANI YASUMASA 独立行政法人国立病院機構(名古屋医療センター臨床研究センター), その他部局等, その他 (90303403)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | ウイルス / 生体分子 / 酵素 / タンパク質 / 生体防御因子 / レトロウイルス / 宿主因子 / APOBEC3 / 感染制御 / シチジンデアミナーゼ / 核酸結合 / 生化学 / HIV / 核酸結合タンパク質 / 生化学的解析 |
Outline of Final Research Achievements |
Human APOBEC3 family proteins are powerful cellular defense factors that potently inhibit replication of retroviruses, including HIV and retrotransposons. However, some retroviruses such as HIV replicate by expressing Vif protein, which specifically eliminates the APOBEC3s' antiviral functions. In this research project, we obtained two major findings: 1) the antiviral molecular mechanisms are fundamentally linked to their homo-oligomerization capacity, which is directly correlated with affinity (dissociation constants) to single-stranded nucleic acids; 2) the Vif-binding conformations on APOBEC3C/F/D are characterized as shallow hydrophobic cavities surrounded with negatively-charged area. These data provide important and novel information to further understand the molecular mechanisms of APOBEC3s' antiviral functions.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Oligomerization transforms human APOBEC3G from an efficient enzyme to a slowly dissociating nucleic acid binding protein.2014
Author(s)
Chaurasiya KR, McCauley MJ, Wang W, Qualley DF, Wu T, Kitamura S, Geertsema H, Chan DSB, Hertz A, Iwatani Y, Levin JG, Musier-Forsyth K, Rouzina I, and Williams MC.
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Journal Title
Nature Chemistry
Volume: 6
Issue: 1
Pages: 28-33
DOI
Related Report
Peer Reviewed
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[Journal Article] Lack of Association between Intact/Deletion Polymorphisms of the APOBEC3B Gene and HIV-1 Risk.2014
Author(s)
Imahashi M, Izumi T, Watanabe D, Imamura J, Matsuoka K, Ode H, Masaoka T, Sato K, Kaneko N, Ichikawa S, Koyanagi Y, Takaori-Kondo A, Utsumi M, Yokomaku Y, Shirasaka T, Sugiura W, Iwatani Y, Naoe T.
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Journal Title
PLoS One
Volume: 9
Issue: 3
Pages: e92861-e92861
DOI
Related Report
Peer Reviewed
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[Journal Article] Poly-proline motif in HIV-2 Vpx is critical for its efficient translation2014
Author(s)
Ariko Miyake, Mikako Fujita, Haruna Fujino, Ryoko Koga, Sogo Kawamura, Masami Otsuka, Hirotaka Ode, Yasumasa Iwatani, Yosuke Sakai, Naoya Doi, Masako Nomaguchi, Akio Adachi, Yasuyuki Miyazaki
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Journal Title
Journal of General Virology
Volume: 95
Issue: 1
Pages: 179-189
DOI
Related Report
Peer Reviewed
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[Journal Article] Results of external quality assessment for proviral DNA testing of HIV tropism in the Maraviroc Switch collaborative study.2013
Author(s)
Tu E, Swenson LC, Land S, Pett S, Emery S, Marks K, Kelleher AD, Kaye S, Kaiser R, Schuelter E, Harrigan R and the research laboratory.
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Journal Title
Journal of Clinical Microbiology
Volume: 51
Issue: 7
Pages: 2063-2071
DOI
Related Report
Peer Reviewed
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[Journal Article] TRIM5 genotypes in cynomolgus monkeys primarily influence inter-individual diversity in susceptibility to monkey-tropic human immunodeficiency virus type 12013
Author(s)
Saito A, Nomaguchi M, Kono K, Iwatani Y, Yokoyama M, Yasutomi Y, Sato H, Shioda T, Sugiura W, Matano T, Adachi A, Nakayama E, Akari H
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Journal Title
J Gen Virol
Volume: 94(Pt 6)
Issue: 6
Pages: 1318-24
DOI
Related Report
Peer Reviewed
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[Journal Article] Generation of rhesus macaque-tropic HIV-1 clones that are resistant to major anti-HIV-1 restriction factors2013
Author(s)
Nomaguchi M, Yokoyama M, Kono K, Nakayama EE, Shioda T, Doi N, Fujiwara S, Saito A, Akari H, Miyakawa K, Ryo A, Ode H, Iwatani Y, Miura T, Igarashi T, Sato H, Adachi A
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Journal Title
J Virol
Volume: 87(21)
Issue: 21
Pages: 11447-11461
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular epidemiology of HIV-1 infection in Iran: genomic evidence of CRF35_AD predominance and CRF01_AE infection among individuals associated with injection drug use.2012
Author(s)
Jahanbakhsh F, Ibe S, Hattori J, Monavari SHR, Matsuda M, Maejima M, Iwatani Y, Memarnejadian A, Keyvani H, Azadmanesh K, Sugiura W
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Journal Title
AIDS Research and Human Retroviruses
Volume: 29
Issue: 1
Pages: 198-203
DOI
Related Report
Peer Reviewed
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[Journal Article] Association of APOBEC3G genotypes and CD4 decline in Thai and Cambodian HIV-infected children with moderate immune deficiency.2012
Author(s)
Bunupuradah T, Imahashi M, Iampornsin T, Matsuoka K, Iwatani Y, Puthanakit T, Ananworanich J, Sophonphan J, Mahanontharit A, Naoe T, Vonthanak S, Phanuphak P, Sugiura W, et al.
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Journal Title
AIDS Research and therapy
Volume: 9
Issue: 1
Pages: 34-34
DOI
Related Report
Peer Reviewed
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[Journal Article] The APOBEC3C crystal structure and the interface for HIV-1 Vif binding.2012
Author(s)
Kitamura S, Ode H, Nakashima M, Imahashi M, Naganawa Y, Kurosawa T, Yokomaku Y, Yamane T, Watanabe N, Suzuki A, Sugiura W, Iwatani Y
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Journal Title
Nature Structural and Molecular Biology
Volume: 19
Issue: 10
Pages: 1005-1010
DOI
Related Report
Peer Reviewed
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[Presentation] Crystal structure of the Vif-inteaction domain of the anti-viral APOBE3F2014
Author(s)
Nakashima M, Kitamura S, Kurosawa T, Ode H, Kawamura T, Imahashi Y, Yokomaku Y, Watanabe N, Sugiura W, Iwatani Y
Organizer
23rd Congress and general assembly of the international union of crystallography
Place of Presentation
Cold Spring Harbor, NY, USA
Year and Date
2014-08-05 – 2014-08-12
Related Report
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[Presentation] Lack of Association between Intact/Deletion Polymorphisms of the APOBEC3B Gene and HIV-1 Risk2014
Author(s)
Imahashi M, Izumi T, Imamura J, Matsuoka K, Ode H, Masaoka T, Sato K, Koyanagi Y, Takaori-Kondo A, Yokomaku Y, Sugiura W, Iwatani Y
Organizer
Cold Spring Harbor Laboratory Annual meeting on Retroviruses
Place of Presentation
Cold Spring Harbor, NY, USA
Year and Date
2014-05-19 – 2014-05-24
Related Report
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[Presentation] Fine-tuned HIV-1 Vif-interaction interface of anti-retroviral cytidine deaminase APOBEC3F2014
Author(s)
Nakashima M, Kitamura S, Kurosawa T, Ode H, Kawamura T, Mano Y, Naganawa Y, Yokomaku Y, Watanabe N, Sugiura W, Iwatani Y
Organizer
Cold Spring Harbor Laboratory Annual meeting on Retroviruses
Place of Presentation
Cold Spring Harbor, NY, USA
Year and Date
2014-05-19 – 2014-05-24
Related Report
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[Presentation] Crystal structure of human APOBEC3C and HIV-1 Vif-binding interface2013
Author(s)
Kitamura S, Ode H, Nakashima M, Imahashi M, Naganawa Y, Kurosawa T, Yokomaku Y, Yamane T, Watanabe N, Suzuki A, Sugiura W, Iwatani Y
Organizer
American Crystallographic Association Annual Meeting
Place of Presentation
ハワイ、米国
Related Report
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[Presentation] ヒト抗レトロウイルス因子APOBEC3ファミリー間におけるHIV-1 Vif 結合インターフェイスの構造比較2013
Author(s)
北村紳悟, 大出裕高, 中島雅晶, 今橋真弓, 長縄由里子, 黒沢哲平, 横幕能行, 山根隆, 渡邉信久, 鈴木淳巨, 杉浦亙, 岩谷靖雅
Organizer
第13回日本蛋白質科学会年会
Place of Presentation
鳥取
Related Report
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[Presentation] The crystal structure of APOBEC3C including HIV-1 Vif-binding interface2013
Author(s)
Kitamura S, Ode H, Nakashima M, Imahashi M, Naganawa Y, Kurosawa T, Yokomaku Y, Yamane T, Watanabe N, Suzuki A, Sugiura W, Iwatani Y
Organizer
4th International Symposium on Diffraction Structural Biology
Place of Presentation
名古屋
Related Report
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[Presentation] The APOBEC3C crystal structure and the interface for HIV-1 Vif interaction (Talk)2012
Author(s)
Kitamura S, Ode H, Nakashima M, Imahashi M, Naganawa Y, Yokomaku Y, Suzuki A, Watanabe N, Sugiura W, Iwatani Y
Organizer
Cold Spring Harbor Laboratory Meeting (2012 meeting on Retroviruses)
Place of Presentation
Cold Spring Harbor, NY, USA
Related Report
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[Presentation] Conformational conservation of the HIV-1 Vif-binding interface of APOBEC3C, DE, F. (Poster)2012
Author(s)
Kitamura S, Ode H, Nakashima M, Imahashi M, Naganawa Y, Yokomaku Y, Suzuki A, Watanabe N, Sugiura W, Iwatani Y
Organizer
Cold Spring Harbor Laboratory Meeting (2012 meeting on Retroviruses)
Place of Presentation
Cold Spring Harbor, NY, USA
Related Report
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[Presentation] Oligomerization of HIV-1 restriction factor APOBEC3G transforms it from a fast enzyme to a slow nucleic acid binding protein. (Talk)2012
Author(s)
Chaurasiya KR, Geertsema H, Qualley DF, Wu T, Iwatani Y, Chan D, Hertz A, Levin JG, Musier-Forsyth K, Rouzina I, Williams MC
Organizer
Cold Spring Harbor Laboratory Meeting (2012 meeting on Retroviruses)
Place of Presentation
Cold Spring Harbor, NY, USA
Related Report
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