| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Human APOBEC3 family proteins are powerful cellular defense factors that potently inhibit replication of retroviruses, including HIV and retrotransposons. However, some retroviruses such as HIV replicate by expressing Vif protein, which specifically eliminates the APOBEC3s' antiviral functions. In this research project, we obtained two major findings: 1) the antiviral molecular mechanisms are fundamentally linked to their homo-oligomerization capacity, which is directly correlated with affinity (dissociation constants) to single-stranded nucleic acids; 2) the Vif-binding conformations on APOBEC3C/F/D are characterized as shallow hydrophobic cavities surrounded with negatively-charged area. These data provide important and novel information to further understand the molecular mechanisms of APOBEC3s' antiviral functions.
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