Functional analysis of neurite outgrowth inhibitor proteins in B cell activation

• Project/Area Number: 24590572
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Immunology
• Research Institution: Tohoku University
• Principal Investigator: INUI Masanori 東北大学, 加齢医学研究所, 講師 (80443985)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: B細胞 / 免疫寛容 / TLR / 自己寛容 / 免疫制御 / 自己免疫疾患

Outline of Final Research Achievements

In this study, I examined the physiological roles of neurite outgrowth inhibitor protein Nogo and Nogo receptor (NgR1) in B cell activation. Nogo-deficient B cells showed a significant reduction of IL-6 and IgM production in response to LPS, CpG and poly(I:C). Thus, Nogo was indispensable for full activation of nucleic acid-sensing TLR responses in B cells.

Research Products

• [Journal Article] Dichotomy in the FcγRIIB deficiency and autoimmune-prone SLAM haplotype clarifies the roles of the Fc receptor in development of autoantibodies and glomerulonephritis. (2014)
  • Author(s): Kanari Y, Sugahara–Tobinai A, Takahashi H, Inui M, Nakamura A, Hirose S, Takai T.
  • Journal Title: BMC Immunol Volume: 15 Issue: 1 Pages: 47-47
  • DOI: 10.1186/s12865-014-0047-y
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 自己免疫病とPirB (2013)
  • Author(s): 乾 匡範，高井俊行
  • Journal Title: 医学のあゆみ Volume: 245 Pages: 225-228
• [Journal Article] Intravenous immunoglobulin suppresses IL-10 production by activated B cells in vitro (2012)
  • Author(s): Tanaka J
  • Journal Title: Open J. Immunol. Volume: vol. 2 Issue: 04 Pages: 149-160
  • DOI: 10.4236/oji.2012.24019
  • Peer Reviewed
• [Journal Article] 破骨細胞共刺激受容体による正と負の制御 (2012)
  • Author(s): 乾 匡範，高井俊行
  • Journal Title: 炎症と免疫 Volume: 20 Pages: 240-245
• [Journal Article] ペア型受容体による破骨細胞の分化制御 (2012)
  • Author(s): 乾 匡範
  • Journal Title: 医学のあゆみ Volume: 242 Pages: 660-664
• [Journal Article] NogoおよびMHC class IによるPirBを介した新たなマスト細胞の制御機構 (2012)
  • Author(s): 乾 匡範
  • Journal Title: 臨床免疫・アレルギー科 Volume: 58 Pages: 185-189
• [Journal Article] 免疫グロブリン様受容体による破骨細胞の分化制御 (2012)
  • Author(s): 乾 匡範，高井俊行
  • Journal Title: CLINICAL CALCIUM Volume: 22 Pages: 1651-1657
• [Presentation] 血小板を介する新たな炎症制御機構の同定 (2014)
  • Author(s): 乾 匡範，田澤樹乃，岸 義朗，髙井俊行
  • Organizer: 日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-17
• [Presentation] ヒト末梢血CD20+CD27+CD43+ B細胞はプラズマブラストよりもメモリーB細胞に近い特徴を有する (2014)
  • Author(s): 乾 匡範，髙井俊行
  • Organizer: 日本炎症・再生医学会
  • Place of Presentation: 沖縄
  • Year and Date: 2014-07-02
• [Presentation] 抗ヒト血小板抗体による炎症応答の制御機構 (2013)
  • Author(s): 乾 匡範，岸 義朗，高井俊行
  • Organizer: 日本炎症・再生医学会
  • Place of Presentation: 京都
• [Presentation] Nogoタンパク質によるTLRシグナルと炎症応答遺伝子の発現制御機構 (2013)
  • Author(s): 木村俊文，乾 匡範，高井俊行
  • Organizer: 日本炎症・再生医学会
  • Place of Presentation: 京都
• [Presentation] Identification of a novel regulatory pathway of inflammation via platelets (2013)
  • Author(s): Inui M, Tazawa K, Kishi Y, Takai T
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 幕張
• [Presentation] Modulation of Toll-like receptor signaling by Nogo in macrophages (2013)
  • Author(s): Kimura T, Inui M, Takai T
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 幕張
• [Presentation] 健常ヒト末梢血中に同定された新しいCD43陽性 B細胞の性状解析 (2013)
  • Author(s): 弘田紗瑛子，乾 匡範，高井俊行
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Regulation of TLR signaling by a reticulon family protein Nogo in macrophages. (2012)
  • Author(s): Kimura T, Inui M, Takai T
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 神戸
• [Presentation] Essential roles of CCL3-CCR1 axis in the pathogenesis of antigen-induced arthritis. (2012)
  • Author(s): Ishida Y, Inui M, Kimura A, Nosaka M, Kuninaka Y, Mukaida N, Kondo T
  • Organizer: 日本免疫学会学術集会
  • Place of Presentation: 神戸