| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
Bcl6, a sequence specific transcriptional repressor, is important for generation and maintenance of central memory CD8+ T cells. However, the molecular mechanism involved in the generation is largely unknown. We demonstrated that Bcl6 negatively regulates the in vivo differentiation of both activated CD25+ CD8+ T cells and KLRG1hi effector CD8+ T cells. Since the induction of CD25 expression was more rapid and the amount of phosphorylated STAT5 was higher in the in vitro activated Bcl6-deficient CD8+ T cells than in the activated wild type CD8+ T cells, the IL-2 signaling was negatively regulated in activated CD8+ T cells by Bcl6. We also confirmed the Bcl6 binding to the various regions in the CD25 and IL-2 genes. These results indicate that Bcl6 is responsible for the differentiation of memory precursor CD8+ T cells by regulating the IL-2 signaling in activated CD8+ T cells.
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