• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of newly identified nucleic acid-recognition molecules in innate immunity

Research Project

Project/Area Number 24590574
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Immunology
Research InstitutionThe University of Tokyo

Principal Investigator

YANAI Hideyuki  東京大学, 生産技術研究所, 准教授 (70431765)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords自然免疫 / シグナル伝達 / サイトカイン / 核酸 / ノックアウトマウス / 自然免疫系 / 核酸認識受容体 / 感染 / ウイルス / 細菌 / 病原体
Outline of Final Research Achievements

Pathogen infection induces robust innate immune responses. Nucleic acids from pathogens such as viruses and bacteria are recognized by nucleic acid-recognition receptors and evokes the activation of innate immune responses. We searched for host cell molecules which are involved in the responses and identified two RNA-binding motif-containing molecules, namely NAS1 and NAS2. In this research we established conditional knockout mice lines of NAS1 and NAS2 and analyzed the contribution of NAS1 and NAS2 to nucleic acid-mediated innate immune responses. Interestingly, we have found that the activation of type I IFN genes and IL-12p40 gene by B-DNA or CpG-B stimulation were impaired in NAS1 KO dendritic cells. In addition, NAS1 KO mice were easily succumbed to HSV-1 infection and Listeria monocytegenes infection. These results indicate that NAS1 is critical to host defense against pathogen infection.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (17 results)

All 2015 2014 2013 2012 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (5 results) (of which Invited: 3 results) Remarks (3 results)

  • [Journal Article] Recognition of tumor cells by Dectin-1 orchestrates innate immune cells for anti-tumor responses.2014

    • Author(s)
      Chiba S, Ikushima H, Ueki H, Yanai H, Kimura Y, Hangai S, Nishio J, Negishi H, Tamura T, Saijo S, Iwakura Y, Taniguchi T.
    • Journal Title

      eLife

      Volume: 04177 Pages: 04177-04177

    • DOI

      10.7554/elife.04177

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Nucleic acid sensing and beyond: virtues and vices of HMGB1.2014

    • Author(s)
      Yanai H, Taniguchi T.
    • Journal Title

      J. Intern. Med.

      Volume: 276 Issue: 5 Pages: 444-453

    • DOI

      10.1111/joim.12285

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Conditional ablation of HMGB 1 in mice reveals its protective function a gainst endotoxemia and bacterial infection2013

    • Author(s)
      Yanai, H, et al.
    • Journal Title

      Proc. Natl. Acad. Sci. U S A.

      Volume: 51 Issue: 51 Pages: 20699-20704

    • DOI

      10.1073/pnas.1320808110

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Beneficial innate signaling interference for anti-bacterial responses by a TLR-mediated enhancement of the MKP-IRF3 axis2013

    • Author(s)
      Negishi H, Matsuki K, Endo N, Sarashina H. Miki S, Matsuda A, Fukazawa K, Taguchi-Atarashi N, Ikushima H, Yanai H, Nishio J, Honda K, Fujioka Y, Ohba Y, Noda T, Taniguchi S, Nishida E, Zhang Y, Chi H, Flavell RA, Taniguchi T
    • Journal Title

      Proc Nat1 Acad Sci U S A

      Volume: 49 Issue: 49 Pages: 19884-19889

    • DOI

      10.1073/pnas.1320145110

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulation of cooperative function of the I112b enhancer and promoter by the interferonre Kulatorv factors 3 and 52013

    • Author(s)
      Koshiba, R., et al.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 430 Issue: 1 Pages: 95-100

    • DOI

      10.1016/j.bbrc.2012.11.006

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] High-mobility group box family of proteins: ligand and sensor for innate immunity.2012

    • Author(s)
      Hideyuki Yanai
    • Journal Title

      Trends in Immunology

      Volume: 33 Issue: 12 Pages: 633-640

    • DOI

      10.1016/j.it.2012.10.005

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cross-interference of RLR and TLR signaling pathways modulates antibacterial T cellresponses2012

    • Author(s)
      Negishi, H., et al.
    • Journal Title

      Nature Immunology

      Volume: 13 Issue: 7 Pages: 659-666

    • DOI

      10.1038/ni.2307

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Essential contribution of IRF3 to intestinal homeostasis and microbiota-mediated Tslp gene induction2012

    • Author(s)
      Hideo Negishi, Shoji Miki, Hana Sarashina, Naoko Taguchi-Atarashi, Akira Nakajima, Kosuke Matsuki, Nobuyasu Endo, Hideyuki Yanai, Junko Nishio, Kenya Honda, and Tadatsugu Taniguchi
    • Journal Title

      PNAS

      Volume: 109(51) Issue: 51 Pages: 21016-21021

    • DOI

      10.1073/pnas.1219482110

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The IRF family of transcription factors: Inception, impact and implications in oncogenesis.2012

    • Author(s)
      Hideyuki Yanai
    • Journal Title

      Oncoimmunology

      Volume: 1 Pages: 1376-1386

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 自然免疫受容体Dectin-1の抗腫瘍応答における役割2015

    • Author(s)
      柳井秀元
    • Organizer
      日本癌学会シンポジウム/共同利用共同研究拠点シンポジウム
    • Place of Presentation
      金沢
    • Year and Date
      2015-01-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Recognition of tumor cells by Dectin-1 induces NK cell-dependent anti-tumor responses2014

    • Author(s)
      Hideyuki Yanai
    • Organizer
      第14回日独がんワークショップ
    • Place of Presentation
      ドイツ、ベルリン
    • Year and Date
      2014-11-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 自然免疫応答・炎症性疾患におけるHMGB1の役割2014

    • Author(s)
      柳井秀元
    • Organizer
      第87回 日本生化学会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Role of HMGB1 in inflammatory diseases and nuclei acid-mediated immune responses2014

    • Author(s)
      Hideyuki Yanai
    • Organizer
      The American Association of Immunologists (AAI)
    • Place of Presentation
      アメリカ合衆国、ピッツバーグ
    • Year and Date
      2014-05-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 自然免疫応答・炎症性疾患におけるHMGB1の役割2012

    • Author(s)
      柳井秀元
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Remarks] 東京大学生産技術研究所 炎症免疫制御学社会連携研究部門

    • URL

      http://www.iis.u-tokyo.ac.jp/~mol-immu/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 東京大学生産技術研究所 炎症・免疫制御学社会連携研究部門

    • URL

      http://www.iis.u-tokyo.ac.jp/~mol-immu/

    • Related Report
      2013 Research-status Report
  • [Remarks] 炎症・免疫制御学社会連携研究部門

    • URL

      http://www.iis.u-tokyo.ac.jp/̃mol-immu/index.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi