| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Pathogen infection induces robust innate immune responses. Nucleic acids from pathogens such as viruses and bacteria are recognized by nucleic acid-recognition receptors and evokes the activation of innate immune responses. We searched for host cell molecules which are involved in the responses and identified two RNA-binding motif-containing molecules, namely NAS1 and NAS2. In this research we established conditional knockout mice lines of NAS1 and NAS2 and analyzed the contribution of NAS1 and NAS2 to nucleic acid-mediated innate immune responses. Interestingly, we have found that the activation of type I IFN genes and IL-12p40 gene by B-DNA or CpG-B stimulation were impaired in NAS1 KO dendritic cells. In addition, NAS1 KO mice were easily succumbed to HSV-1 infection and Listeria monocytegenes infection. These results indicate that NAS1 is critical to host defense against pathogen infection.
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