Analysis of newly identified nucleic acid-recognition molecules in innate immunity
| Project/Area Number |
24590574
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YANAI Hideyuki 東京大学, 生産技術研究所, 准教授 (70431765)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 自然免疫 / シグナル伝達 / サイトカイン / 核酸 / ノックアウトマウス / 自然免疫系 / 核酸認識受容体 / 感染 / ウイルス / 細菌 / 病原体 |
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| Outline of Final Research Achievements |
Pathogen infection induces robust innate immune responses. Nucleic acids from pathogens such as viruses and bacteria are recognized by nucleic acid-recognition receptors and evokes the activation of innate immune responses. We searched for host cell molecules which are involved in the responses and identified two RNA-binding motif-containing molecules, namely NAS1 and NAS2. In this research we established conditional knockout mice lines of NAS1 and NAS2 and analyzed the contribution of NAS1 and NAS2 to nucleic acid-mediated innate immune responses. Interestingly, we have found that the activation of type I IFN genes and IL-12p40 gene by B-DNA or CpG-B stimulation were impaired in NAS1 KO dendritic cells. In addition, NAS1 KO mice were easily succumbed to HSV-1 infection and Listeria monocytegenes infection. These results indicate that NAS1 is critical to host defense against pathogen infection.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Recognition of tumor cells by Dectin-1 orchestrates innate immune cells for anti-tumor responses.2014
Author(s)
Chiba S, Ikushima H, Ueki H, Yanai H, Kimura Y, Hangai S, Nishio J, Negishi H, Tamura T, Saijo S, Iwakura Y, Taniguchi T.
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Journal Title
eLife
Volume: 04177
Pages: 04177-04177
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Beneficial innate signaling interference for anti-bacterial responses by a TLR-mediated enhancement of the MKP-IRF3 axis2013
Author(s)
Negishi H, Matsuki K, Endo N, Sarashina H. Miki S, Matsuda A, Fukazawa K, Taguchi-Atarashi N, Ikushima H, Yanai H, Nishio J, Honda K, Fujioka Y, Ohba Y, Noda T, Taniguchi S, Nishida E, Zhang Y, Chi H, Flavell RA, Taniguchi T
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Journal Title
Proc Nat1 Acad Sci U S A
Volume: 49
Issue: 49
Pages: 19884-19889
DOI
Related Report
Peer Reviewed
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[Journal Article] Essential contribution of IRF3 to intestinal homeostasis and microbiota-mediated Tslp gene induction2012
Author(s)
Hideo Negishi, Shoji Miki, Hana Sarashina, Naoko Taguchi-Atarashi, Akira Nakajima, Kosuke Matsuki, Nobuyasu Endo, Hideyuki Yanai, Junko Nishio, Kenya Honda, and Tadatsugu Taniguchi
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Journal Title
PNAS
Volume: 109(51)
Issue: 51
Pages: 21016-21021
DOI
Related Report
Peer Reviewed
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