maintenance and activation mechanisms of memory B cells
| Project/Area Number |
24590575
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
ADACHI Takahiro 東京医科歯科大学, 難治疾患研究所, 准教授 (50222625)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | B細胞 / カルシウムシグナル / シグナル伝達 / 生体イメージング / 免疫記憶 / 免疫応答 / Ｂ細胞 |
|---|
| Outline of Final Research Achievements |
Ca2+ signaling is one of the typical phenomenon mediated through immune receptors. Analyses of their signaling together with dynamics in vivo is required for understanding of the precise immune responses.I established stable transgenic mice with the genetically encoded Ca2+ indicator yellow cameleon 3.60 (YC3.60) based on the Cre/loxP system. We successfully generated the specific YC3.60 expression mice in immune cells or nerve cells, as well as the ubiquitous expression mice. After crossing with CD19-Cre mice, most B cells exhibited robust YC3.60 expression without significantly altering B-cell function. Intravital imaging of spleen, Peyer’s patches, and bone marrow of the CD19-Cre/YC3.60 mice revealed Ca2+ flux in B cells. B cells exhibiting high Ca2+ were drastically increased in autoimmune-prone CD22 deficient mice. Furthermore, in another autoimmune-prone model C57BL/6-lpr/lpr mouse, lymphocytes of high Ca2+ concentration were increased.
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Report
(5 results)
Research Products
(11 results)
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[Journal Article] Intravital imaging of Ca(2+) signals in lymphocytes of Ca(2+) biosensor transgenic mice: indication of autoimmune diseases before the pathological onset.2016
Author(s)
Yoshikawa S, Usami T, Kikuta J, Ishii M, Sasano T, Sugiyama K, Furukawa T, Nakasho E, Takayanagi H, Tedder TF, Karasuyama H, Miyawaki A, Adachi T.
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Journal Title
Sci Rep. 2016 Jan 6;6:18738.
Volume: 6
Issue: 1
Pages: 18738-18738
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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