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Analysis of tissue macrophage development and its role in vivo

Research Project

Project/Area Number 24590584
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Immunology
Research InstitutionOsaka University

Principal Investigator

Kohyama Masako  大阪大学, 微生物病研究所, 助教 (70311339)

Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords組織マクロファージ / 分化 / Development
Outline of Final Research Achievements

Splenic red pulp macrophages (RPM) degrade senescent erythrocytes and recycle heme-associated iron. The transcription factor SPI-C is selectively expressed by RPM and is required for their development, but the physiologic stimulus inducing Spic is unknown. We found that Spic regulated the development of bone marrow macrophages (BMM) and that Spic expression was induced by heme. Spic expression in monocytes was constitutively inhibited by the transcriptional repressor BACH1. These findings are the first example of metabolite-driven differentiation of a tissue-resident macrophage subset and provide new insights into iron homeositasis. Hmox1 (HO-1) is the enzyme that catabolizes free heme into biliverdin, iron, and CO. To analyze the function of RPM in malaria parasite clearance, we infected HO-1 conditional KO mice that lack RPM. Through the analysis of this cKO mice, we found unexpected role of RPM in parasite clearance.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (28 results)

All 2016 2015 2014 2013 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Acknowledgement Compliant: 3 results,  Open Access: 4 results) Presentation (15 results) Remarks (3 results)

  • [Journal Article] Monocyte infiltration into obese and fibrilized tissues is regulated by PILRα.2016

    • Author(s)
      Kohyama M, Matsuoka S, Shida K, Sugihara F, Aoshi T, Kishida K, Ishii KJ, Arase H.
    • Journal Title

      Eur J Immunol.

      Volume: in press Issue: 5 Pages: 1214-1223

    • DOI

      10.1002/eji.201545897

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] RAG-mediated DNA double-strand breaks activate a cell type-specific checkpoint to inhibit pre-B cell receptor signals.2016

    • Author(s)
      Bednarski JJ, Pandey R, Schulte E, White LS, Chen BR, Sandoval GJ, Kohyama M, Haldar M, Nickless A, Trott A, Cheng G, Murphy KM, Bassing CH, Payton JE, Sleckman BP.
    • Journal Title

      RAG-mediated DNA double-strand breaks activate a cell type-specific checkpoint to inhibit pre-B cell receptor signals.

      Volume: 213 Issue: 2 Pages: 209-223

    • DOI

      10.1084/jem.20151048

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sialic Acids on Varicella-Zoster Virus Glycoprotein B Are Required for Cell-Cell Fusion.2015

    • Author(s)
      Suenaga T, Matsumoto M, Arisawa F, Kohyama M, Hirayasu K, Mori Y, Arase H.
    • Journal Title

      J Biol Chem.

      Volume: 290 Issue: 32 Pages: 19833-19843

    • DOI

      10.1074/jbc.m114.635508

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] β2-glycoprotein I/ HLA class II complexes are novel autoantigens in antiphospholipid syndrome.2015

    • Author(s)
      Tanimura K, Jin H, Suenaga T, Morikami S, Arase N, Kishida K, Hirayasu K, Kohyama M, Ebina Y, Yasuda S, Horita T, Takasugi K, Ohmura K, Yamamoto K, Katayama I, Sasazuki T, Lanier LL, Atsumi T, Yamada H, Arase H.
    • Journal Title

      Blood

      Volume: 125 Issue: 18 Pages: 2835-2844

    • DOI

      10.1182/blood-2014-08-593624

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Negative regulation of DSS-induced experimental colitis by PILRα.2015

    • Author(s)
      Kishida, K., Kohyama, M., Kurashima, Y., Kogure, Y., Wang, J., Hirayasu, K., Suenaga, T., Kiyono, H., Kunisawa, J. and Arase, H.
    • Journal Title

      Int. Immunol.

      Volume: - Issue: 6 Pages: 307-314

    • DOI

      10.1093/intimm/dxv004

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Engineering large viral DNA genomes using the CRISPR-Cas9 system.2014

    • Author(s)
      Suenaga T, Kohyama M, Hirayasu K, Arase H.
    • Journal Title

      Microbiol Immunol.

      Volume: 58(9) Issue: 9 Pages: 513-522

    • DOI

      10.1111/1348-0421.12180

    • NAID

      40020207309

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Olfactory plays a key role in spatiotemporal pathogenesis of cerebral malaria.2014

    • Author(s)
      H. Zhao, T. Aoshi, S. Kawai, Y. Mori, A. Konishi, M. Ozkan, Y. Fujita, Y. Haseda, M. Shimizu, M. Kohyama, K. Kobiyama, K. Eto, J. Nabekura, T. Horii, T. Ishino, M. Yuda, H. Hemmi, T. Kaisho, S. Akira, M. Kinoshita, K. Tohyama, Y. Yoshioka, K. J. Ishii, C. Coban
    • Journal Title

      Cell Host Microbe

      Volume: 15 Issue: 5 Pages: 551-563

    • DOI

      10.1016/j.chom.2014.04.008

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Heme-Mediated SPI-C Induction Promotes Monocyte Differentiation into Iron-Recycling Macrophages.2014

    • Author(s)
      Haldar, M., Kohyama, M., So, A.Y., Kc, W., Wu, X., Briseno, C.G., Satpathy, A.T., Kretzer, N.M., Arase, H., Rajasekaran, N.S., Wang, L., Egawa, T., Igarashi, K., Baltimore, D., Murphy, T.L., and Murphy, K.M.
    • Journal Title

      Cell

      Volume: 156 Issue: 6 Pages: 1223-1234

    • DOI

      10.1016/j.cell.2014.01.069

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Autoantibodies to IgG/HLA class II complexes are associated with rheumatoid arthritis susceptibility2014

    • Author(s)
      Jin H, Arase N, Hirayasu K, Kohyama M, Suenaga T, Saito F, Tanimura K, Matsuoka S, Ebina K, Shi K, Toyama-Sorimachi N, Yasuda S, Horita T, Hiwa R, Takasugi K, Ohmura K, Yoshikawa H, Saito T, Atsumi T, Sasazuki T, Katayama I, Lanier LL, Arase H
    • Journal Title

      Proceedings of National Academy of Science USA

      Volume: 111 Issue: 10 Pages: 3787-3792

    • DOI

      10.1073/pnas.1401105111

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transport of misfolded endoplasmic reticulum proteins to the cell surface by MHC class II molecules.2013

    • Author(s)
      Jiang, Y., Arase, N., Kohyama, M., Hirayasu, K., Suenaga, T., Jin, H., Matsumoto, M., Shida, K., L. Lanier, L., Saito, T. and Arase, H.
    • Journal Title

      Int. Immunol.

      Volume: 25 Issue: 4 Pages: 235-246

    • DOI

      10.1093/intimm/dxs155

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Myeloperoxidase/HLA classII complex are targets for autoantibodies in ANCA-associated vasculitis2015

    • Author(s)
      Hiwa Ryosuke, Ohmura Koichiro, Arase Noriko, Jin Hui, Hirayasu Kouyuki, Kohayama Masako, Suenaga Tadahiro, Saito Fumiji, Iwatani Hirotsugu, Atsumi Tatsuya, Terao Chikashi, Mimori Tsuneyo, Arase Hisashi
    • Organizer
      第44日本免疫学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2015-11-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] PILRα negatively regulates monocyte infiltration into the skin2015

    • Author(s)
      Kohyama Masako, Matsuoka Sumiko, Arase Noriko, Arase Hisashi
    • Organizer
      第44日本免疫学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2015-11-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] MHC class II molecules expose autoantibody epitopes on autoantigens2015

    • Author(s)
      Jin Hui, Arase Noriko, Matsuoka Sumiko, Hirayasu Kouyuki, Kohayama Masako, Suenaga Tadahiro, Sasazuki Takehiko, Arase Hisashi
    • Organizer
      第44日本免疫学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2015-11-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 食餌鉄依存的な腸管CX3CR1high制御性ミエロイド細胞維持機構2015

    • Author(s)
      香山尚子, 香山雅子, 竹田潔
    • Organizer
      第43回日本臨床免疫学会総会
    • Place of Presentation
      神戸国際会議場(兵庫)
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] MPO/HLA classⅡ複合体は顕微鏡的多発血管炎における自己抗体の標的である2015

    • Author(s)
      日和良介, 大村浩一郎, 荒瀬規子, 金暉, 平安恒幸, 香山雅子, 末永忠広, 齋藤史路, 岩谷博次, 渥美達也, 寺尾知可史, 三森経世, 荒瀬尚
    • Organizer
      第43回日本臨床免疫学会総会
    • Place of Presentation
      神戸国際会議場(兵庫)
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Myeloperoxidase/HLA class II complexes are targets for autoantibodies in microscopic polyangiitis.2014

    • Author(s)
      Hiwa Ryosuke, Ohmura Koichiro, Arase Noriko, Jin Hui, Hirayasu Kouyuki, Kohayama Masako, Suenaga Tadahiro, Matsuoka Sumiko, Iwatani Hirotsugu, Atsumi Tatsuya, Terao Chikashi, Mimori Tsuneyo, Arase Hisashi
    • Organizer
      第43回日本免疫学会学術集会
    • Place of Presentation
      国立京都国際会館(京都)
    • Year and Date
      2014-12-10 – 2014-12-12
    • Related Report
      2014 Research-status Report
  • [Presentation] PILRα negatively regulates size of adipose tissue by controlling monocyte mobility2014

    • Author(s)
      Kohyama Masako, Kishida Kazuki, Arase Hisashi
    • Organizer
      第43回日本免疫学会学術集会
    • Place of Presentation
      国立京都国際会館(京都)
    • Year and Date
      2014-12-10 – 2014-12-12
    • Related Report
      2014 Research-status Report
  • [Presentation] MHC class II-restricted recognition of self-antigen/MHC class II complexes by autoantibodies2014

    • Author(s)
      Jin Hui, Arase Noriko, Matsuoka Sumiko, Hirayasu Kouyuki, Kohayama Masako, Suenaga Tadahiro, Nakamaru Yuji, Imatani Yoshinori, Katayama Ichiro, Arase Hisashi
    • Organizer
      第43回日本免疫学会学術集会
    • Place of Presentation
      国立京都国際会館(京都)
    • Year and Date
      2014-12-10 – 2014-12-12
    • Related Report
      2014 Research-status Report
  • [Presentation] Autoantibodies in Rheumatoid Arthritis Specifically Recognize IgG Heavy Chain Complexed with HLA-DR, Which is Strongly Associated with Rheumatoid Arthritis Susceptibility.2014

    • Author(s)
      Jin H, Arase N, Hirayasu K, Kohyama M, Suenaga T, Saito F, Tanimura K, Matsuoka S, Ebina K, Shi K, Toyama-Sorimachi N, Yasuda S, Horita T, Hiwa R, Takasugi K, Ohmura K, Yoshikawa H, Saito T, Atsumi T, Sasazuki T, Katayama I, Lanier LL, Arase H.
    • Organizer
      The 15th Annual European Congress of Rheumatology EULAR 2014
    • Place of Presentation
      Paris(France)
    • Year and Date
      2014-06-11 – 2014-06-14
    • Related Report
      2014 Research-status Report
  • [Presentation] MHCクラスⅡ分子によって細胞外へ輸送された細胞内ミスフォールド蛋白質が自己抗体の標的分子である2014

    • Author(s)
      金暉、荒瀬規子、平安恒幸、香山雅子、末永忠広、松岡須美子、斉藤隆、Lewis L. Lanier、荒瀬尚
    • Organizer
      第24回Kyoto T cell Conference
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2014-05-16 – 2014-05-17
    • Related Report
      2014 Research-status Report
  • [Presentation] Rheumatoid factor binding to IgG heavy chain presented on HLA-DR is associated with Rheumatoid Arthritis susceptibility

    • Author(s)
      Jin Hui, Arase Noriko, Kohayama Masako, Saito Fumiji, Hirayasu Kouyuki, Matsumoto Maki, Shida Kyoko, Suenaga Tadahiro, Saito Takashi, Katayama Ichiro, Lanier Lewis L., Arase Hisashi
    • Organizer
      第42回日本免疫学会学術集会
    • Place of Presentation
      幕張メッセ(千葉市)
    • Related Report
      2013 Research-status Report
  • [Presentation] β2-glycoprotein I presented on MHC class II molecules are recognized by autoantibodies in antiphospholipid syndrome

    • Author(s)
      Tanimura Kenji, Suenaga Tadahiro, Jin Hui, Hirayasu Kouyuki, Arase Noriko, Kohayama Masako, Ebina Yasuhiko, Yasuda Shinsuke, Horita Tetsuya, Katayama Ichiro, Atsumi Tatsuya, Yamada Hideo, Arase Hisashi
    • Organizer
      第42回日本免疫学会学術集会
    • Place of Presentation
      幕張メッセ(千葉市)
    • Related Report
      2013 Research-status Report
  • [Presentation] PILRα negatively regulates DSS induced experimental colitis

    • Author(s)
      Kishida Kazuki, Kohyama Masako, Kurashima Yosuke, Wang Jing, Hirayasu Kouyuki, Suenaga Tadahiro, Kiyono Hiroshi, Kunisawa Jun, Arase Hisashi
    • Organizer
      第42回日本免疫学会学術集会
    • Place of Presentation
      幕張メッセ(千葉市)
    • Related Report
      2013 Research-status Report
  • [Presentation] PILRα negatively regulates monocyte mobility

    • Author(s)
      Kohyama Masako, Wang Jing Kishida Kazuki, Arase Hisashi
    • Organizer
      第42回日本免疫学会学術集会
    • Place of Presentation
      幕張メッセ(千葉市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Transport of misfolded ER proteins to the cell surface by MHC class II molecules

    • Author(s)
      Jiang, Y., Arase, N., Kohyama, M., Hirayasu, K., Suenaga, T., Jin, H., Matsumoto, M., Shida, K., L. Lanier, L., Saito, T. and Arase, H.
    • Organizer
      第41回日本免疫学会学術集会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Related Report
      2012 Research-status Report
  • [Remarks] 免疫化学分野ホームページ

    • URL

      http://immchem.biken.osaka-u.ac.jp/

    • Related Report
      2014 Research-status Report
  • [Remarks] 免疫化学分野ホームページ

    • URL

      http://immchem.biken.osaka-u.ac.jp/

    • Related Report
      2013 Research-status Report
  • [Remarks] 大阪大学微生物病研究所免疫化学分野

    • URL

      http://immchem.biken.osaka-u.ac.jp/

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2020-03-17  

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