| Project/Area Number |
24590610
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical sociology
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| Research Institution | Kobe University |
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Principal Investigator |
OSAWA KAYO 神戸大学, 保健学研究科, 准教授 (50324942)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Hiroyuki 神戸大学, 医学部附属病院, 感染制御部 (40626248)
SHIRAKAWA Toshiro 神戸大学, 大学院保健学研究科, 教授 (70335446)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 細菌 / 抗菌薬 / 薬剤耐性 / 分子疫学 / メタロβラクタマーゼ / 基質特異性拡張型βラクタマーゼ / βラクタマーゼ / ホッジテスト / シークエンスタイピング / パルスフィールドゲル電気泳動法 |
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| Outline of Final Research Achievements |
The spread of β-lactamases that hydrolyze βlactam antibiotics has limited options for treating bacterial infections.The prevance of
metallo-β-lactamase (MBL) producing Enterobacteriae has been incresing worldwide. In this study, we investigated the molecular characteristics of 200 strains of Salmonella, 74 strains of Escherichia coli, and 35 strains of Enterobacteriae with resistance for calbapenem antibiotics. We performed the antimicrobial susceptibility tests and detected β lactamase related genes, and epidemiology typing by PCR and sequencing technique. Two strains of Salmonella and 72 strains of Escherichia coli has extended-spectrum β-lactamase (ESBL), with resistance for major β lactam antibiotics, related genes represented a majority worldwide. Of 35 strains in Enterobacteriae with resistance for calbapenem, 17 strains has MBL related genes. In conclusion, we confirmed that ESBL and MBL producing Enterobacteriae has emerged in Japan.
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