| Project/Area Number |
24590680
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Azabu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NOMURA Fumio 千葉大学, 医学(系)研究科(研究院), 教授 (80164739)
SATOH Mamoru 千葉大学, 医学部附属病院, 寄附研究部門教員 (20401002)
SATO Kenichi 国際医療福祉大学, 保健医療学部, 講師 (90505687)
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| Co-Investigator(Renkei-kenkyūsha) |
KODERA Yoshio 北里大学, 理学部, 准教授 (60265733)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 病原微生物 / 細菌同定 / MALDI-TOF MS / プロテオミクス / 病原微生物同定 |
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| Outline of Final Research Achievements |
MALDI-TOF MS is increasingly used in clinical microbial diagnostics for species identification of pathogens. The identification rates at the species and genus levels were 91.7% and 97.0%, respectively, and both rates were improved by adding clinical isolate data obtained in our laboratory to that in the original commercial database.
MRSA is one of the major pathogens responsible for nosocomial infection. The presence of MRSA in a hospital is detrimental to patients and to hospital management. Thus, rapid identification of MRSA is needed. Here, we report a prospective study of rapid discrimination of MSSA from MRSA using MALDI-TOF MS in 160 clinical isolates of S. aureus. The predictive model was trained using 50 MSSA and 50 MRSA. The identification rates were 90.0% for MSSA and 87.5% for MRSA. In blind test sets, 30 MSSA and 30 MRSA were correctly classified, with identification rates of 93.3% for MSSA and 86.7% for MRSA.
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