Development of a redox biological reaction-based diagnostic method at bedside for oxidative stress
| Project/Area Number |
24590686
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | University of Fukui |
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Principal Investigator |
KASUNO Kenji 福井大学, 医学部, 准教授 (60455243)
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| Co-Investigator(Kenkyū-buntansha) |
IWANO Masayuki 福井大学, 医学部, 教授 (20275324)
KIMURA Hideki 福井大学, 医学部附属病院, 准教授 (20283137)
TKAHASHI Naoki 福井大学, 医学部附属病院, 助教 (30377460)
NAKAMURA Hajime 公益財団法人田附興風会, 医学研究所第8研究部, 研究員 (70303914)
木村 秀樹 福井大学, 医学部附属病院, 准教授 (20283187)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 酸化ストレス / 急性腎障害 / 尿中チオレドキシン / チオレドキシン |
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| Outline of Final Research Achievements |
We found that urinary thioredoxin (TRX) levels were markedly higher in patients with acute kidney injury (AKI) than in those with chronic kidney disease or in healthy subjects. In a steady state, TRX in blood is filtered and reabsorbed by renal tubular cells. During kidney injury, tubular cells excrete TRX in an oxidative form in response to oxidative stress. With using this unique phenomenon, we developed a redox biological reaction-based diagnostic method at bedside for oxidative stress. This method employed chemiluminescent immuno assay, and completed within 15 minutes. For the differentiation between AKI and other renal diseases, the area under the curve for urinary TRX was 0.94 (95% confidence interval, 0.90-0.98) in a receiver operating characteristic curve analysis, and the sensitivity and specificity were 0.88 and 0.88, respectively, at the optimal cutoff value of 43.0 ug/g creatinine. This diagnostic ability is comparable to established AKI markers NGAL, KIM-1, and L-FABP.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] A heterozygous female with Fabry disease due to a novel α-galactosidase A mutation exhibits a unique synaptopodin distribution in vacuolated podocytes.2015
Author(s)
Takahashi N, Yokoi S, Kasuno K, Kogami A, Tsukimura T, Togawa T, Saito S, Ohno K, Hara M, Kurosawa H, Hirayama Y, Kurose T, Yokoyama Y, Mikami D, Kimura H, Naiki H, Sakuraba H, Iwano M.
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Journal Title
Clin Nephrol.
Volume: 83
Issue: 05
Pages: 301-308
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Severe intraglomerular detachment of podocytes in a Gitelman syndrome patient2012
Author(s)
Naoki Takahashi, Hideki Kimura, Shinichi Mizuno, Masanori Hara, Yoshiaki Hirayama, Hiroyuki Kurosawa, Shouji Tsutaya, Minoru Yasujima, Seiji Yokoi, Daisuke Mikami, Kenji Kasuno, Hironobu Naiki, Masayuki Iwano and Haruyoshi Yoshida
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Journal Title
Clin Exp Nephrol
Volume: (in press)
Issue: 3
Pages: 16-16
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] 新規尿中バイオマーカー2015
Author(s)
糟野健司
Organizer
第58回（平成27年）日本腎臓学会学術総会
Place of Presentation
名古屋国際会議場
Year and Date
2015-06-05 – 2015-06-07
Related Report
Invited
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[Presentation] 低酸素ストレス性腎障害マーカーとしての尿中ポドカリキシン濃度ーdb/dbマウスを用いてー2013
Author(s)
高橋 直生, 糟野 健司, 上山 和子, 黒澤 寛之, 平山 吉朗, 黒瀬 知美, 横井 靖二, 横山 由就, 三上 大輔, 木村 秀樹, 吉田 治義, 原 正則, 岩野 正之
Organizer
日本腎臓学会学術総会
Place of Presentation
東京国際フォーラム
Related Report
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