Molecular analysis of pathogenesis and the unique machinery of cell division in Helicobacter pylori
| Project/Area Number |
24590697
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KUMON Yoshio 高知大学, 教育研究部医療学系, 准教授 (40215033)
SUGIURA Tetsuro 高知大学, 教育研究部医療学系, 教授 (50171145)
MORIMOTO Norihito 高知大学, 医学部附属病院, その他 (60398055)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | ヘリコバクター・ピロリ / 細胞分裂制御システム / 形態形成 / minCDE / ftsZ / cdrA / coccoid / 細胞分裂 / CdrA / FtsZ / minシステム / 分子相互作用 / Minシステム |
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| Outline of Final Research Achievements |
Bacterial cells precisely divide, coordinated by many molecular components including Min proteins and FtsZ. However, the role of Min proteins in H. pylori is little known. We investigated the function of Min proteins with a wild-type HPK5 and HPK5-derivative mutants constructed. All disruptants were filamentous with few minicells. These genes maintained the cell morphology. The lowest coccoid form appeared in minE-disruptant, indicating that MinE contributes to the coccoid conversion at the stationary phase. FtsZ was dispersed throughout a cell in only minD-disruptant by immunofluorescence microscopy, revealing that MinD is involved in conduct of FtsZ localization. These showed the intrinsic characteristics of H. pylori Min proteins and provided new insights to understand the cell division of H. pylori.
Furthermore, we first discovered new spherical phages (KHP30 and KHP40) from 2 clinical isolates.
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Report
(4 results)
Research Products
(69 results)
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[Presentation] Evaluation of Helicobacter pylori genotyping by DiversiLab system based on rep-PCR2014
Author(s)
Morimoto N, Takeuchi H, Nishida Y, Morisawa M, Yoshikawa T, Morita T, Morimoto M, Sugimoto C, Matsumura Y, Sugiura T.
Organizer
The 9th Cherry Blossom Symposium
Place of Presentation
Yokohama, Japan (Yokohama Red Brick Warehouse)
Year and Date
2014-04-17 – 2014-04-19
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[Presentation] Estimated distribution of KHP30-like bacteriophages in the stomach, using clinical Helicobacter pylori isolates2014
Author(s)
Jumpei Uchiyama, Hiroaki Takeuchi, Yoshihiko Sakaguchi, Iyo Takemura-Uchiyama, Shin-ichiro Kato, Keiji Gamoh, Takako Ujihara, Masanori Daibata, Shigenobu Matsuzaki
Organizer
The 12th Japan-Korea International Symposium on Microbiology
Place of Presentation
Tower Hall Funabori, Tokyo
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[Presentation] A novel type of spherical bacteriophage, Helicobacter pylori bacteriophage KHP302013
Author(s)
Uchiyama J, Takeuchi H, Sakaguchi Y, Gamo K, Kato S, Takemura-Uchiyama I, Ujihara T, Daibata M, Matsuzaki S.
Organizer
5th Congress of European Microbiologists
Place of Presentation
Leipzing,Germany
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[Presentation] Superoxide dismutase (SOD) of Helicobacter pylori clinical isolates from patients with gastroduodenal diseases2012
Author(s)
Morishita K, Takeuchi H, Shimamura T, Morimoto N, Kadota Y, Tsuda M, Taniguchi T, Ukeda H, Sugiura T,Yamamoto T
Organizer
112th ASM General Meeting
Place of Presentation
Convention center, (San Francisco, USA）
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[Book] Nutrition and Food Sciences2012
Author(s)
Trang VT, Thanh LX, Takeuchi H
Total Pages
256
Publisher
International Association of Computer Science & Information Technology Singapore
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