Development of early diagnostic markers for aging-related peripheral neuropathy and novel anti-aging therapies.

• Project/Area Number: 24590699
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kumamoto University
• Principal Investigator: OBAYASHI KONEN 熊本大学, 大学院生命科学研究部, 教授 (90361899)
• Co-Investigator(Kenkyū-buntansha): ANDO Yukio 熊本大学, 大学院生命科学研究部, 教授 (20253742)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ニューロパチー / 加齢変化 / アンチエイジング

Outline of Final Research Achievements

In this study, we tried to develop early diagnostic markers for aging-related peripheral neuropathy and novel anti-aging therapies. Our results suggest that urinary liver-type fatty acid binding protein, quantitative sensory testing(QST), serum or urinary Glycer-glyceraldehyde-derived advanced glycation end product (Glycer-AGEs), small-fiber pain threshold values, and amount of the deposition of alpha-synuclein in peripheral nerves are the most useful methods to diagnose the aging-related peripheral neuropathy. Our present finding may have some implications for developing novel anti-aging therapies.

Research Products

• [Journal Article] Measurement of small-fiber pain threshold values for the early detection of diabetic polyneuropathy. (2015)
  • Author(s): Kukidome D, Nishikawa T, Sato M, Igata M, Kawashima J, Shimoda S, Matsui K, Obayashi K, Ando Y, Araki E.
  • Journal Title: Diabet Med Volume: in press
  • Peer Reviewed
• [Journal Article] Amyloid deposits derived from transthyretin in the ligamentum flavum as related to lumbar spinal canal stenosis. (2015)
  • Author(s): Yanagisawa A, Ueda M, Sueyoshi T, Okada T, Fujimoto T, Ogi Y, Kitagawa K, Tasaki M, Misumi Y, Oshima T, Jono H, Obayashi K, Hirakawa K, Uchida H, Westermark P, Ando Y, Mizuta H.
  • Journal Title: Mod Pathol Volume: 28 Issue: 2 Pages: 201-207
  • DOI: 10.1038/modpathol.2014.102
  • Peer Reviewed
• [Journal Article] Clinico-histopathological and biochemical analyses of corneal amyloidosis in gelatinous drop-like corneal dystrophy. (2015)
  • Author(s): Tasaki M, Ueda M, Matsumoto K, Kawaji T, Misumi Y, Eiki D, Suenaga G, Obayashi K, Yamashita T, Tanihara H, Ando Y.
  • Journal Title: Amyloid Volume: 22 Issue: 1 Pages: 67-69
  • DOI: 10.3109/13506129.2014.983222
  • Peer Reviewed
• [Journal Article] Development of the enzymatic assay for whole blood choline using an automated biochemical analysis. (2014)
  • Author(s): Yumura A, Hokazono E, Ikeda K. Obayashi K, Ando Y, Osawa S.
  • Journal Title: Int J Anal Bio-Sci Volume: 2 Pages: 23-32
  • Peer Reviewed / Open Access
• [Journal Article] Changes in pathological and biochemical findings of systemic tissue sites in familial amyloid polyneuropathy more than 10 years after liver transplantation (2013)
  • Author(s): Oshima T, Kawahara S, Ueda M, Kawakami Y, Tanaka R, Okazaki T, Misumi Y, Obayashi K, Yamashita T, Ohya Y, Ihse E, Shinriki S, Tasaki M, Jono H, Asonuma K, Inomata Y, Westermark P, Ando Y
  • Journal Title: J Neurol Neurosurg Psychiatry Volume: - Issue: 7 Pages: 740-746
  • DOI: 10.1136/jnnp-2013-305973
  • Peer Reviewed
• [Journal Article] Dominant cardiac type of familial amyloidotic polyneuropathy associated with a novel transthyretin variant Thr59Arg (2013)
  • Author(s): Obayashi K, Misumi Y, Watanabe T, Ando T, Akagami T, Tasaki M, Shinriki S, Ueda M, Yamashita T, Hirotani S, Ando Y
  • Journal Title: Muscle Nerve Volume: 48(5) Issue: 5 Pages: 839-41
  • DOI: 10.1002/mus.23939
  • Peer Reviewed
• [Journal Article] Salivary mental stress proteins. (2013)
  • Author(s): Obayashi K.
  • Journal Title: Clin Chim Acta Volume: 425 Pages: 196-201
  • DOI: 10.1016/j.cca.2013.07.028
  • Peer Reviewed
• [Journal Article] Solitary amyloidosis of sigmoid colon featuring submucosal tumor caused hematochezia: Case report. (2013)
  • Author(s): Ogasawara N, Kitagawa W, Obayashi K, et al.
  • Journal Title: Intern Med Volume: 52 Pages: 2523-2527
  • Peer Reviewed
• [Journal Article] Usefulness of B-type natriuretic peptide as a marker of cardiac diastolic function in senile systemic amyloidosis. (2013)
  • Author(s): Usuku H, Obayashi K, Shono M, et al.
  • Journal Title: Amyloid Volume: 20 Issue: 4 Pages: 251-255
  • DOI: 10.3109/13506129.2013.844122
  • Peer Reviewed
• [Journal Article] Effect of age and sex differences on wild-type transthyretin amyloid formation in familial amyloidotic polyneuropathy: a proteomic approach (2013)
  • Author(s): Tasaki M, Ueda M, Obayashi K, Koike H, Kitagawa K, Ogi Y, Jono H, Su Y, Suenaga G, Oshima T, Misumi Y, Yoshida M, Yamashita T, Sobue G, Ando Y
  • Journal Title: Int J Cardiol Volume: 170 Issue: 1 Pages: 69-74
  • DOI: 10.1016/j.ijcard.2013.10.033
  • Peer Reviewed
• [Journal Article] Impact of antibodies against amyloidogenic transthyretin (ATTR) on phenotypes of patients with familial amyloidotic polyneuropathy (FAP) ATTR Valine30Methionine (2013)
  • Author(s): Obayashi K, Tasaki M, Jono H, Ueda M, Shinriki S, Misumi Y, Yamashita T, Oshima T, Nakamura T, Ikemizu S, Anan I, Suhr O, Ando Y
  • Journal Title: Clin Chim Acta Volume: 419 Pages: 127-31
  • DOI: 10.1016/j.cca.2013.02.002
  • Peer Reviewed
• [Journal Article] Loss of gastric interstitial cells of Cajal in patients with hereditary transthyretin amyloidosis. (2013)
  • Author(s): Wixner J, Obayashi K, Ando Y, Karling P, Anan I.
  • Journal Title: Amyloid Volume: in press
  • Peer Reviewed
• [Journal Article] Peritransplant gastrointestinal symptoms in familial amyloidotic polyneuropathy. (2013)
  • Author(s): Ohya Y, Isono K, Obayashi K, Hayashida S, Lee KJ, Yamamoto H, Takeichi T, Asonuma K, Ando Y, Inomata Y.
  • Journal Title: Exp Clin Transplant Volume: in press Issue: 4 Pages: 327-331
  • DOI: 10.6002/ect.2012.0257
  • Peer Reviewed
• [Journal Article] Pathological changes long after liver transplantation in a familial amyloidotic polyneuropathy patient. (2012)
  • Author(s): Obayashi K, Ueda M, Oshima T, Kawahara S, Misumi Y, Yamashita T, Jono H, Yazaki M, Kametani F, Ikeda S, Ohya Y, Asonuma K, Inomata Y, Ando Y.
  • Journal Title: BMJ Case Rep Volume: bcr2012006593
  • Peer Reviewed
• [Journal Article] Interaction between amyloid fibril formation and extracellular matrix in the proceedings of VIIIth International Symposium on Familial Amyloidotic Polyneuropathy. (2012)
  • Author(s): Misumi Y
  • Journal Title: Amyloid Volume: 19 Suppl 1 Issue: sup1 Pages: 8-10
  • DOI: 10.3109/13506129.2012.674987
  • Peer Reviewed
• [Presentation] 多角的自律神経機能検査法を用いた FAP ATTR V30Mの早期診断 (2014)
  • Author(s): 大林光念、安東由喜雄
  • Organizer: 第67回日本自律神経学会教育講演
  • Place of Presentation: ラフレさいたま(さいたま市)
  • Year and Date: 2014-10-30 – 2014-10-31
  • Invited
• [Presentation] 多角的自律神経機能検査法を用いた小径線維ニューロパチーの診断 (2014)
  • Author(s): 大林光念、安東由喜雄
  • Organizer: 第55回日本神経学会学術大会シンポジウム
  • Place of Presentation: 福岡国際会議場 (福岡市)
  • Year and Date: 2014-05-21 – 2014-05-24
  • Invited
• [Presentation] Transthyertin familial amyloidotic polyneuropathy (TTR-FAP): Pathogenesis, clinical features, diagnosis, and therapies. (2013)
  • Author(s): Obayashi K.
  • Organizer: 9th Japan-China Cooperative Life Science Symposium
  • Place of Presentation: 山東大学 (中国山東省済南)
  • Invited
• [Presentation] 栄養評価と炎症マーカー (2013)
  • Author(s): 大林光念、安東由喜雄
  • Organizer: 第53回日本臨床化学会年次学術集会
  • Place of Presentation: あわぎんホール (徳島市)
  • Invited
• [Presentation] FAP患者の胃内におけるCajal 細胞、粘膜内小径線維の密度とアミロイド沈着パターンとの関連 (2013)
  • Author(s): 大林光念, 大嶋俊範, 田崎雅義, 山下太郎, 安東由喜雄.
  • Organizer: 第54回日本神経学会学術集会
  • Place of Presentation: 東京国際フォーラム (東京都千代田区)
• [Presentation] 全身性アミロイドーシスの診断と自律神障害. (2012)
  • Author(s): 大林光念、山下太郎、大嶋俊範、安東由喜雄.
  • Organizer: 第65回日本自律神経学会総会.
  • Place of Presentation: 都市センターホテル
• [Presentation] 家族性アミロイドポリニューロパチー患者における異型トランスサイレチンに対する自己抗体の存在とその意義. (2012)
  • Author(s): 大林光念、田崎雅義、神力悟、植田光晴、城野博史、安東由喜雄.
  • Organizer: 第52回日本臨床化学会年次集会.
  • Place of Presentation: いわて県民情報交流センター
• [Presentation] C線維の機能評価にAδ特異的痛覚閾値検査を組み合わせた小径線維ニューロパチーの早期診断. (2012)
  • Author(s): 大林光念、大嶋俊範、山下太郎、安東由喜雄.
  • Organizer: 第23回日本末梢神経学会学術集会.
  • Place of Presentation: 九州大学医学部百年講堂
• [Presentation] Aδ特異的痛覚閾値検査とC線維の機能評価を組み合わせた小径線維ニューロパチーの早期診断法に関する検討. (2012)
  • Author(s): 大林光念、植田光晴、三隅洋平、大嶋俊範、山下太郎、安東由喜雄.
  • Organizer: 第53回日本神経学会学術集会.
  • Place of Presentation: 東京国際フォーラム
• [Presentation] Detection of autoantibodies against ATTR in patients with FAP ATTR V30M. (2012)
  • Author(s): Obayashi K, Oshima T, Tasaki M, Suhr OB, Anan I, Misumi Y, Ueda M, Yamashita T, Jono H, Ando Y.
  • Organizer: XIIＩth International Symposium on Amyloidosis
  • Place of Presentation: Groningen, Netherlands
• [Book] 心に残る経腸栄養の患者さんたち (2015)
  • Author(s): 大林光念
  • Total Pages: 377
  • Publisher: フジメディカル出版
• [Book] 南山堂医学大辞典 (2015)
  • Author(s): 大林光念
  • Total Pages: 3101
  • Publisher: 南山堂
• [Book] 臨床検査: 診療ガイドラインに活用される臨床検査: 神経・筋領域 (2014)
  • Author(s): 大林光念
  • Total Pages: 6
  • Publisher: 医学書院
• [Book] 臨床検査: NSTの機能を高める検査室間連携 (2014)
  • Author(s): 大林光念, 安東由喜雄
  • Total Pages: 6
  • Publisher: 医学書院
• [Book] 臨床検査: 生殖細胞系遺伝子検査(遺伝学的検査） (2013)
  • Author(s): 大林光念, 安東由喜雄
  • Total Pages: 6
  • Publisher: 医学書院
• [Book] 臨床検査 (2013)
  • Author(s): 大林光念、安東由喜雄
  • Total Pages: 5
  • Publisher: 医学書院
• [Remarks] 熊本大学大学院保健学研究部
  • URL: http://www.hs.kumamoto-u.ac.jp/
• [Remarks] 熊本大学医学部附属病院中央検査部アミロイドーシス診療体制構築事業
  • URL: http://www2.kuh.kumamoto-u.ac.jp/amyloidunit/