| Project/Area Number |
24590702
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Chiba Institute of Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMURA Satoshi 千葉科学大学, 危機管理学部, 講師 (30406805)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 赤血球膜タンパク / Na+/K+-ATPase / ナトリウムポンプ / HPLC / C12E8 / なし |
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| Outline of Final Research Achievements |
To isolate high-purity erythrocyte membrane-derived Na+/K+-ATPase, WGA-agarose column chromatography was used to remove impurities. The target protein was obtained as a peak eluted by 0.03 M GlcNAc. To verify the identity of this protein, the subunit structure of Na+/K+-ATPase was analyzed by SDS-PAGE. We confirmed the presence of bands corresponding to a molecular weight of approximately 97kDa by Silver impregnation stain. Further, we compared the erythrocyte membrane-derived protein with the Na+/K+-ATPase isolated and purified from pig kidney. As a result the positions of elution peak was corresponding. Thus, we successfully established a simple method to isolate Na+/K+-ATPase from erythrocyte membrane.
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