Development of ultra high speed and highly sensitive simultaneous bio- and chemi-luminescent assay and its application to multi labeled immunoassay.
| Project/Area Number |
24590707
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Showa University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 生物発光 / 化学発光 / 超高速高感度測定 / イクオリン / ガウシアルシフェラーゼ / アクリニジウムエステル / マルチラベルイムノアッセイ / 多成分同時測定 / 超高速高感度 / 3成分同時測定 |
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| Outline of Final Research Achievements |
I have developed simultaneous bio- and chemi- luminometric assay using aequorin (AQ), gaussia luciferase (GL) and acrinidium ester (AE). In this study, we applied this analytical method to the detection of muliplex-labeled immunoassay. Minimum detection limit of AQ, GL and AE were 6.4 x 10-20, 9.7 x10-19 and 6.5 x 10-19 mol/assay utilizing proposed simultaneous bio- and chemi- luminometric assay, respectively. I also applied to multiplex-labeled immunoassay for tumor makers and mice cytokines. These measurable ranges were 4.0～263 ng/mL (AFP), 8.8～565 ng/mL (PSA) and 2.8～4620 ng/mL (CEA) for human tumor makers and 0.625-40ng/mL (TNF-α), 20-1250pg/mL (IFN-γ), 20-1250pg/mL (IL-6) for mice cytokines, respectively.
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Report
(4 results)
Research Products
(2 results)
