Profile analysis of disease-related markers in synovial tissues of rheumatoid arthritis using imaging mass spectrometry

• Project/Area Number: 24590714
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Osaka Medical College
• Principal Investigator: TAKEUCHI Tooru 大阪医科大学, 医学部, 講師 (10330078)
• Co-Investigator(Kenkyū-buntansha): NAKANISHI Toyofumi 大阪医科大学, 医学部, 准教授 (10247843) ; MAKINO Shigeki 大阪医科大学, 医学部, 教授 (20268204) ; KOTANI Takuya 大阪医科大学, 医学部, 助教 (80411362)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 関節リウマチ / プロテオミクス / 質量イメージング / 関節炎 / 質量分析

Outline of Final Research Achievements

This paper showed that the infiltration of inflammatory cells such as macrophages and lymphocytes into synovitis lesions and the expression of TGF-β, VEGF, MMP-3 and -9 in arthritis-model mice are elevated in comparison to control mice. Proteomics-based quantitative analysis showed the elevated expression of α-enolase and other unidentified markers in synovial tissues of arthritis. Tissue sections for imaging mass spectrometry could not obtained by microtome because of bone tissues and small samples.