Study on novel mechanism for TRPV1-mediated control of neuropathic pain

• Project/Area Number: 24590734
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pain science
• Research Institution: Nigata University of Phermacy and Applied Life Sciences
• Principal Investigator: TAKEHIKO Maeda 新潟薬科大学, 薬学部, 教授 (50271010)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 神経障害性疼痛 / 骨髄移植 / 骨髄キメラマウス / カプサイシン / TRPV1 / ノックアウトマウス / 触アロディニア

Outline of Final Research Achievements

We studied mechanisms for TRPV1-mediated control of neuropathic pain in mice. We found recruitment of immune cells and differentiation and hypertrophy of adipocyte in the injured sciatic nerve tissue. Stimulation of TRPV1 on immune cells inhibited the development of neuropathic pain. Interaction of immune cells and adipocytes in the injured nerve tissue facilitates the development of neuropathic pain, revealed by gene expression profiling in co-culture of immune cells and adipocytes. These studies suggest that TRPV1 on immune cells is a target molecule for the control of neuropathic pain.

Research Products

• [Journal Article] Peripheral interleukin-4 ameliorates inflammatory macrophage-dependent neuropathic pain. (2015)
  • Author(s): Kiguchi N, Kobayashi Y, Saika F, Sakaguchi H, Maeda T, Kishioka S
  • Journal Title: Pain Volume: 156(4) Issue: 4 Pages: 684-93
  • DOI: 10.1097/j.pain.0000000000000097
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Study on Novel Mechanism Underlying Analgesia Targeting TRPV1 (2014)
  • Author(s): Maeda T, Ozaki M.
  • Journal Title: YAKUGAKU ZASSHI Volume: 134 Issue: 3 Pages: 373-378
  • DOI: 10.1248/yakushi.13-00236-1
  • NAID: 130003391179
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2014-03-01
  • Peer Reviewed
• [Journal Article] Possible Involvement of Endogenous Opioid System Located Downstream of <i>α</i>7 Nicotinic Acetylcholine Receptor in Mice With Physical Dependence on Nicotine (2014)
  • Author(s): Ueno K1, Kiguchi N, Kobayashi Y, Saika F, Wakida N, Yamamoto C, Maeda T, Ozaki M, Kishioka S.
  • Journal Title: Journal of Pharmacological Sciences Volume: 124 Issue: 1 Pages: 47-53
  • DOI: 10.1254/jphs.13172FP
  • NAID: 130003382624
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Journal Article] HMGB1 as a Potential Therapeutic Target for Neuropathic Pain (2013)
  • Author(s): Maeda T1, Ozaki M, Kobayashi Y, Kiguchi N, Kishioka S.
  • Journal Title: Journal of Pharmacological Sciences Volume: 123 Issue: 4 Pages: 301-305
  • DOI: 10.1254/jphs.13R08CP
  • NAID: 130003382621
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Journal Article] Activation of nicotinic acetylcholine receptors on bone marrow-derived cells relieves neuropathic pain accompanied by peripheral neuroinflamation. (2012)
  • Author(s): Kiguchi N, Kobayashi Y, Maeda T, Tominaga S, Nakamura J, Fukazawa Y, Ozaki M and Kishioka S
  • Journal Title: Neurochem Int. Volume: 61 Issue: 7 Pages: 1212-1219
  • DOI: 10.1016/j.neuint.2012.09.001
  • Peer Reviewed
• [Journal Article] The critical role of spinal ceramide in the development of partial sciatic nerve ligation-induced neuropathic pain in mice (2012)
  • Author(s): Kobayashi Y, Kiguchi N, Maeda T, Ozaki M, Kishioka S.
  • Journal Title: Biochem Biophys Res Commun Volume: 421 Issue: 2 Pages: 318-322
  • DOI: 10.1016/j.bbrc.2012.03.153
  • Peer Reviewed
• [Journal Article] Epigenetic augmentation of the macrophage inflammatory protein 2/C-X-C chemokine receptor type 2 axis through histone H3 acetylation in injured peripheral nerves elicits neuropathic pain (2012)
  • Author(s): Kiguchi N, et al
  • Journal Title: J Pharmacol Exp Ther Volume: 340 Issue: 3 Pages: 577-587
  • DOI: 10.1124/jpet.111.187724
  • Peer Reviewed
• [Presentation] TRPチャネルを標的とする新規鎮痛機序の解析 (2013)
  • Author(s): 前田武彦
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
  • Invited