Analysis of genetic mechanism for reduced nociceptive response by genome-wide gene expression assay
| Project/Area Number |
24590741
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pain science
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
KASAI Shinya 公益財団法人東京都医学総合研究所, 精神行動医学研究分野, 主席研究員 (20399471)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 疼痛感受性 / 鎮痛薬感受性 / 全ゲノム遺伝子発現 / モデルマウス |
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| Outline of Final Research Achievements |
CXBH mice exhibited slightly higher morphine-induced antinociception compared with C57BL/6J and BALB/cBy mice in the hot-plate test but not tail-flick test. CXBH mice exhibited a marked reduction of nociceptive sensitivity, regardless of the type of nociceptive stimulus, with the exception of tail stimulation. Changes in gene expression that corresponded to reduced nociceptive sensitivity in the brains of CXBH mice were observed in 62 transcripts, including pain- and analgesia-related transcripts, in a whole-genome expression assay. The total mRNA expression of opioid receptors was higher in CXBH mice than in C57BL/6J and BALB/cBy mice. However, the expression levels of MOR-1 mRNA, a major transcript of the μ opioid receptor gene, were not different among the C57BL/6J, BALB/cBy, and CXBH strains. In conclusion, supraspinal nociceptive responses were reduced in the CXBH mouse strain, and the expression levels of transcripts were altered in the brain of this strain.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] Association between the rs1465040 single-nucleotide polymorphism close to the transient receptor potential subfamily C member 3 (TRPC3) gene and postoperative analgesic requirements.2015
Author(s)
Aoki Y, Nishizawa D, Hasegawa J, Kasai S, Yoshida K, Koukita Y, Ichinohe T, Nagashima M, Katoh R, Satoh Y, Tagami M, Hayashida M, Fukuda K, Ikeda K.
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Journal Title
J Pharmacol Sci
Volume: 127
Issue: 3
Pages: 391-393
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Association Between <i>KCNJ6</i> (<i>GIRK2</i>) Gene Polymorphism rs2835859 and Post-operative Analgesia, Pain Sensitivity, and Nicotine Dependence2014
Author(s)
Nishizawa D, Fukuda K, Kasai S, Ogai Y, Hasegawa J, Sato N, Yamada H, Tanioka F, Sugimura H, Hayashida M, Ikeda K.
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Journal Title
Journal of Pharmacological Sciences
Volume: 126
Issue: 3
Pages: 253-263
DOI
NAID
ISSN
1347-8613, 1347-8648
Related Report
Peer Reviewed / Open Access
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[Journal Article] Genome-wide association study identifies a potent locus associated with human opioid sensitivity.2014
Author(s)
Nishizawa D, Fukuda K, Kasai S, Hasegawa J, Aoki Y, Nishi A, Saita N, Koukita Y, Nagashima M, Katoh R, Satoh Y, Tagami M, Higuchi S, Ujike H, Ozaki N, Inada T, Iwata N, Sora I, Iyo M, Kondo N, Won MJ, Naruse N, Uehara-Aoyama K, Itokawa M, Koga M, Arinami T, Kaneko Y, Hayashida M, Ikeda K.
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Journal Title
Mol Psychiatry
Volume: 19(1)
Issue: 1
Pages: 55-62
DOI
Related Report
Peer Reviewed
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[Presentation] Associations of an orexin (hypocretin) receptor 2 gene polymorphism with nicotine dependence found in genome-wide and following association studies2014
Author(s)
Nishizawa D, Kasai S, Hasegawa J, Sato N, Tanioka F, Nagashima M, Ujike H, Hashimoto R, Tanaka M, Sugimura H, Ikeda K
Organizer
29th CINP World Congress of Neuropsychopharmacology
Place of Presentation
Vancouver, Canada
Year and Date
2014-06-23
Related Report
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[Presentation] Genome-wide association study identifies a potent locus associated with human opioid sensitivity.2013
Author(s)
Nishizawa D, Fukuda K, Kasai S, Hasegawa J, Aoki Y, Nishi A, Saita N, Koukita Y, Nagashima M, Katoh R, Satoh Y, Tagami M, Higuchi S, Ujike H, Ozaki N, Inada T, Iwata N, Sora I, Iyo M, Kondo N, Won MJ, Naruse N, Uehara K, Itokawa M, Koga M, Arinami T, Kaneko Y, Hayashida M, Ikeda K.
Organizer
European Human Genetics Conference 2013
Place of Presentation
Paris, France
Related Report
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[Presentation] Genome-wide association study identifies a potent locus associated with human opioid sensitivity.2013
Author(s)
Nishizawa D, Fukuda K, Kasai S, Hasegawa J, Nagashima M, Satoh Y, Higuchi S, Ujike H, Arinami T, Hayashida M, Ikeda K.
Organizer
XXIst World Congress of Psychiatric Genetics
Place of Presentation
Boston, USA
Related Report
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