Development of bioinformatics tool to predict the hepatotoxicity of chemicals

• Project/Area Number: 24590743
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hygiene
• Research Institution: University of Toyama
• Principal Investigator: INADERA Hidekuni 富山大学, 大学院医学薬学研究部(医学), 教授 (10301144)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 化学物質 / 毒性学 / インフォマティクス / トランスクリプトーム

Outline of Final Research Achievements

The development of systems for analyzing global expression patterns by molecular profiling techniques provides opportunities to establish the basis of toxicological responses. The general profile of gene expression provides insights into the molecular mechanisms by which chemicals might exert toxic effects. We developed a customized DNA microarray system and analyzed gene expression changes in response to representative chemicals. In vitro hepatotoxicity prediction systems using primary cultured hepatocytes have been used in this study. We found in vitro toxicity systems cannot fully reflect the in vivo expression profile and cellular function. We tried to establish the bioinformatics tool which can predict the in vivo response by using the transcriptome data of in vitro systems.

Research Products

• [Journal Article] Endoscopic assessment of reflux esophagitis concurrent with hiatal hernia in male Japanese patients with obstructive sleep apnea. (2014)
  • Author(s): Zenda T, Hamazaki K, Oka R, Hagishita T, Miyamoto S, Shimizu J, and Inadera H.
  • Journal Title: Scand J Gastroenterol. Volume: 49 Issue: 9 Pages: 1035-1043
  • DOI: 10.3109/00365521.2014.926984
  • Peer Reviewed
• [Journal Article] Birth cohort study on the effects of desert dust exposure on children’s health: protocol of an adjunct study of the Japan Environment and Children’s Study. (2014)
  • Author(s): Kanatani KT, Adachi Y, Sugimoto N, Noma H, Onishi K, Hamazaki K, Takahashi Y, Ito I, Egawa M, Sato K, Go T, Kurozawa Y, Inadera H, Konishi I, Nakayama T, and Japan Environment and Children`s Study Group.
  • Journal Title: BMJ Open Volume: 4 Issue: 6 Pages: e004863-e004863
  • DOI: 10.1136/bmjopen-2014-004863
  • Peer Reviewed / Open Access
• [Journal Article] Molecular mechanisms of hyperthermia-induced apoptosis enhanced by docosahexaenoic acid: Implication for cancer therapy. (2014)
  • Author(s): Cui ZG, Piao JL, Kondo T, Ogawa R, Tsuneyama K, Zhao QL, Feril LB Jr, Inadera H.
  • Journal Title: Chem Biol Interact Volume: in press Pages: 46-53
  • DOI: 10.1016/j.cbi.2014.03.005
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of hyperthermia-induced apoptosis enhanced by withaferin A. (2014)
  • Author(s): Cui ZG, Piao JL, Rehman MUR, Ogawa R, Li P, Zhao QL, Kondo T, and Inadera H.
  • Journal Title: Eur J Pharmacol. Volume: 723 Pages: 99-107
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of hyperthermia-induced apoptosis enhanced by withaferin A. (2014)
  • Author(s): Cui ZG, Piao JL, Rehman MUR, Ogawa R, Li P, Zhao QL, Kondo T, and Inadera H.
  • Journal Title: Eur. J. Pharmacol. Volume: 723 Pages: 99-107
  • DOI: 10.1016/j.ejphar.2013.11.031
  • Peer Reviewed
• [Journal Article] Developmental origins of obesity and type 2 diabetes: molecular aspects and role of chemicals. (2013)
  • Author(s): Inadera H
  • Journal Title: Environ. Health Prev. Med. Volume: 18 Issue: 3 Pages: 185-197
  • DOI: 10.1007/s12199-013-0328-8
  • NAID: 10031175979
  • Peer Reviewed
• [Journal Article] Abnormalities in the fatty acid composition of the postmortem entorhinal cortex of patients with schizophrenia, bipolar disorder, and major depressive disorder. (2013)
  • Author(s): Hamazaki K, Hamazaki T, and Inadera H.
  • Journal Title: Psychiatry Res. Volume: 210 Issue: 1 Pages: 346-350
  • DOI: 10.1016/j.psychres.2013.05.006
  • Peer Reviewed
• [Journal Article] Fatty acid composition in the postmortem amygdala of patients with shizophrenia, bipolar disorder, and major depressive disorder. (2012)
  • Author(s): Hamazaki K., Hamazaki T., and Inadera H.
  • Journal Title: J. Psychiatr. Res. Volume: 46 Pages: 1024-1028
  • Peer Reviewed
• [Journal Article] GHS促進のための化学物質取扱いマニュアルの実用化に関する調査研究. (2012)
  • Author(s): 稲寺秀邦，浜崎景，鏡森定信，山上孝司，石渡弘一
  • Journal Title: 産業医学ジャーナル Volume: 35 Pages: 60-64
• [Presentation] ドコサヘキサエン酸によるヘムオキシゲナーゼ1誘導とその分子メカニズムの検討 (2014)
  • Author(s): 崔正国，近藤隆，稲寺秀邦
  • Organizer: 第84回日本衛生学会学術総会
  • Place of Presentation: 岡山
  • Year and Date: 2014-05-25 – 2014-05-27
• [Presentation] PM2.5のアレルギー様症状への影響―「黄砂と子どもの健康調査」より (2014)
  • Author(s): 金谷久美子，足立雄一，浜崎景，稲寺秀邦，奥村智憲，大西一成，黒沢洋一，中山健夫
  • Organizer: 第84回日本衛生学会学術総会
  • Place of Presentation: 岡山
  • Year and Date: 2014-05-25 – 2014-05-27
• [Presentation] 抑うつにおけるω3系脂肪酸の役割 (2013)
  • Author(s): 浜崎景，藤田日奈，浜崎智仁，稲寺秀邦
  • Organizer: 第54回日本心身医学会総会・学術講演会
  • Place of Presentation: 福岡
• [Presentation] 環境と健康に係わる調査における複数回調査の回収状況について，子どもの健康と環境に関する全国調査 富山地区での状況 (2013)
  • Author(s): 城川美佳，浜崎景，稲寺秀邦
  • Organizer: 日本行動計量学会第41回大会
  • Place of Presentation: 習志野
• [Presentation] 子どもの健康と環境に関する全国調査（エコチル調査）の進捗状況について (2013)
  • Author(s): 浜崎景，城川美佳，田中朋美，伊藤実香，足立雄一，稲寺秀邦
  • Organizer: 第33回日本川崎病学会・学術集会
  • Place of Presentation: 富山
• [Presentation] 透析患者において血中n-3系多価不飽和脂肪酸が生命予後に及ぼす影響 ～10年の追跡調査より～ (2013)
  • Author(s): 浜崎景，寺島嘉宏，糸村美保，富田新，黒田昌宏，平田仁，浜崎智仁，稲寺秀邦
  • Organizer: 第41回北陸公衆衛生学会
  • Place of Presentation: 富山
• [Presentation] エコチル調査富山ユニットセンターにおける調査票回収状況 (2013)
  • Author(s): 城川美佳，浜崎景，田中朋美，伊藤実香，足立雄一，稲寺秀邦
  • Organizer: 第16回環境ホルモン学会研究発表会
  • Place of Presentation: 東京
• [Presentation] Fatty acid composition in the postmortem entorhinal cortex of patients with schizophrenia, bipolar disorder and major depression. (2012)
  • Author(s): Hamazaki K., Hamazaki T., and Inadera H.
  • Organizer: 10th Congress of the International Society for the Study of Fatty Acids and Lipids.
  • Place of Presentation: Vancouver, Canada.
• [Presentation] Molecular mechanisms involved in the enhancement of hyperthermia- induced apoptosis by docosahexaenoic acid, -implication for cancer therapy-. (2012)
  • Author(s): Cui ZG., Piao JL., and Inadera H.
  • Organizer: The 11th International Congress of Hyperthermic Oncology.
  • Place of Presentation: Kyoto, Japan.