| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
The Arginine catabolic mobile element (ACME), a genomic island identified in the Staphylococci genome, is characteristically found in strain USA300, which is the predominant MRSA clone in the USA and is spreading globally. In the present study, the prevalence of ACME and the genetic diversity of ACME (+) MRSA were investigated for clinical isolates collected in Hokkaido.
The percentage of ACME carriage against the all MRSA isolates significantly increased in the course of study. Increased number of MRSA with the identical genotype as USA300 was observed, which suggests its potential spread in northern Japan. We focused on the ACME (+) MRSA with type II SCCmec (MRSA-II) because MRSA-II is the predominant genotype in Japanese MRSA. The genetic structures of ACME and its flanking region in MRSA-II were determined and novel genetic variants were identified. The similar analysis was carried out on coagulase-negative staphylococci clinical isolates obtained in Hokkaido.
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