Characterization of novel ACME-SCCmec composite Islands in Japanese MRSA isolates: determination of genome structure and application to epidemiological studies.
| Project/Area Number |
24590751
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Nobumichi 札幌医科大学, 医学部, 教授 (80186759)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | ブドウ球菌 / MRSA / ACME / アルギニン代謝 / SCCmec / 表皮ブドウ球菌 / 分子疫学 |
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| Outline of Final Research Achievements |
The Arginine catabolic mobile element (ACME), a genomic island identified in the Staphylococci genome, is characteristically found in strain USA300, which is the predominant MRSA clone in the USA and is spreading globally. In the present study, the prevalence of ACME and the genetic diversity of ACME (+) MRSA were investigated for clinical isolates collected in Hokkaido.
The percentage of ACME carriage against the all MRSA isolates significantly increased in the course of study. Increased number of MRSA with the identical genotype as USA300 was observed, which suggests its potential spread in northern Japan. We focused on the ACME (+) MRSA with type II SCCmec (MRSA-II) because MRSA-II is the predominant genotype in Japanese MRSA. The genetic structures of ACME and its flanking region in MRSA-II were determined and novel genetic variants were identified. The similar analysis was carried out on coagulase-negative staphylococci clinical isolates obtained in Hokkaido.
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Report
(5 results)
Research Products
(20 results)
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[Journal Article] Drug resistance and genetic characteristics of clinical isolates of staphylococci in Myanmar : high prevalence of PVL among methicillin-susceptible Staphylococcus aureus belonging to various sequence types.2016
Author(s)
Aung MS, Zi H, New KM, Maw WW, Aung MT, Min WW, Nyein N, Kawaguchiya M, Urushibara N, Sumi A, Kobayashi N
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Journal Title
New Microbes and New Infections
Volume: 10
Pages: 58-65
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Detection and Genetic Characterization of PVL-Positive ST8-MRSA-IVa and Exfoliative Toxin D-Positive European CA-MRSA-Like ST1931 (CC80) MRSA-IVa Strains in Bangladesh2014
Author(s)
Paul S.K., Ghosh S., Kawaguchiya M., Urushibara N., Hossain M.A., Ahmed S., Mahmud C., Jilani M.S., Haq J.A., Ahmed A.A., Kobayashi N.
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Journal Title
Microbial Drug Resistance
Volume: -
Issue: 4
Pages: 325-336
DOI
Related Report
Peer Reviewed
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