| Project/Area Number |
24590760
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Hygiene
|
|---|
| Research Institution | Seisen University. |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIRATA Takahumi 京都大学, 理学(系)研究科(研究院), 教授 (10251612)
MATSUKAWA Takehisa 順天堂大学, 医学部, 助教 (60453586)
CHIBA Momoko 順天堂大学, 医学部, 客員教授 (80095819)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | レアメタル / セリウム(Ce) / ユーロピウム(Eu) / マウス肺 / 吸入曝露 / レーザーアブレーション-ICPMS / 化学形態 / マッピング / LA-ICPMS |
|---|
| Outline of Final Research Achievements |
Using laser ablation--ICPMS, distributions and chemical forms of two kinds of rare earth elements cerium (Ce) and europium (Eu) in lung of mice were investigated. Femtosecond-laser and excimer laser were compared and the former was selected. Mice were inhaled with oxide particles of Ce or Eu with 1 or 5 μm diameter at 0.15 mg/m3, 7 hours per day, 5 days per week for 4 weeks and were dissected. Ce and Eu mapping images were obtained for 1μm thickness sections of paraffin embedding lung. Ce particles were distributed in pulmonary alveolus or in macrophage. Very low concentration of Ce was observed in the surroundings of blood vessel suggesting the partial dissolution and movement within lung. Eu was distributed as particles with various sizes suggesting that Eu was dissolved more easily than Ce and moved to other organs via blood flow. Distribution of rare earths was not corresponded with those of Cu and Zn. Frozen section preparation was also made for quantitative analysis.
|
