Molecular mechanisms of exacerbation effects on mouse lung fibrosis induced by diesel exhaust particles
Project/Area Number |
24590764
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Hygiene
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Research Institution | Nippon Medical School |
Principal Investigator |
LI YINGJI 日本医科大学, 医学部, 講師 (60350039)
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Co-Investigator(Kenkyū-buntansha) |
KAWADA Tomoyuki 日本医科大学, 大学院医学研究科, 教授 (00224791)
AZUMA Arata 日本医科大学, 医学部, 教授 (10184194)
INAGAKI Hirofumi 日本医科大学, 医学部, 講師 (50213111)
HIRATA Yukiyo 日本医科大学, 医学部, 助教 (40322515)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | EMT / DEP / oxidative stress / 酸化ストレス / ディーゼル排気粒子 / 細胞遊走能 / 細胞内シグナル伝達 / EMT / 細胞遊走 |
Outline of Final Research Achievements |
The accumulation of fibroblasts plays a critical role in the development of pulmonary fibrosis. Epithelial-mesenchymal transition (EMT) process is related with pulmonary fibrosis progression. The current study was designed to explore the role of Diesel exhaust particle (DEP) whether or not induce EMT process in airway epithelial cells by oxidative stress. In the bronchial epithelial cell line, E-cadherin expression was down-regulated and N-cadherin expression was up-regulated by DEP exposure. The EMT cells migration was significantly increased and the antioxidant enzymes mRNA expression was up-regulated by DEP. These changes by DEP exposure were blocked by N-acetylcysteine pretreatment. Our results suggest that DEP may exacerbate the tissue remodeling processes of pulmonary fibrosis by oxidative stress.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Prostaglandin E2 switches from a stimulator to an inhibitor of cell migration after epithelial-to-mesenchymal transition. Prostaglandins Other Lipid Mediat2015
Author(s)
2)Li YJ, Kanaji N, Wang XQ, Sato T, Nakanishi M, Kim M, Michalski J, Nelson AJ, Farid M, Basma H, Patil A, Toews ML, Liu X, Rennard SI.
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Journal Title
Prostaglandins Other Lipid Mediat
Volume: 116-117
Pages: 1-9
DOI
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Peer Reviewed / Open Access
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[Journal Article] EM, EM703 inhibit NF-kB activation induced by oxidative stress from diesel exhaust particle in human bronchial epithelial cells: Importance in IL-8 transcription2013
Author(s)
Li YJ, Shimizu T, Hirata Y, Inagaki H, Takizawa H, Azuma A, Kawada T, Sugawara I, Kudoh S, Sunazuka T, Omura S.
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Journal Title
Pulm Pharmacol Ther
Volume: 26
Issue: 3
Pages: 318-324
DOI
Related Report
Peer Reviewed
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[Presentation] Nrf2欠損マウスにおけるディーゼル排気粒子曝露のブレオマイシン気道炎症病態への影響.2013
Author(s)
李英姫, 清水孝子, 平田幸代, 稲垣弘文, 新海雄介, 武田健, 吾妻安良太, 滝澤始, 山本雅之, 川田智之, 工藤翔二.
Organizer
第53回日本呼吸器学会学術講演会
Place of Presentation
東京国際フォーラム
Related Report
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[Presentation] Diesel exhaust particle induce epithelial-to-mesenchymal transition by oxidative stress in human bronchial epithelial cell2013
Author(s)
Li YJ, Shimizu T, Hirata Y, Inagaki H, Shinkai Y, Takeda K, Azuma A, Takizawa H, Liu X, Kawada T, Kudoh S.
Organizer
23rd European Respiratory Society
Place of Presentation
FIRA BARCELONAConvention Centre de Gran Via Plaza Europa, Barcelona
Related Report
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[Book] PM2.5: Role of Oxidative Stress in Health Effects and Prevention Strategy (Editors: Ying-Ji Li, Ying-Ji Li, Masakazu Umezawa, Hajime Takizawa, Ken Takeda, and Tomoyuki Kawada) ISBN: 978-1-63482-453-82015
Author(s)
Ying-Ji Li, Hajime Takizawa, Arata Azuma, Tomoyuki Kawada (Chapter 16)
Publisher
Nova Science Publishers, Inc. Hauppauge, NY
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