Effects of environmental chemicals on Th17 cells differenciation via aryl hydrocarbon receptor
| Project/Area Number |
24590773
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Hokkaido Institute of Public Health |
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Principal Investigator |
KOJIMA HIROYUKI 北海道立衛生研究所, 食品科学部, 主幹 (10414286)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Shinji 北海道立衛生研究所, 理化学部, 主査 (20414287)
MUROMOTO Ryuta 北海道大学, 大学院薬学研究院, 助教 (30455597)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUDA Tadashi 北海道大学, 大学院薬学研究院, 教授 (20212219)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 環境化学物質 / AhR / IL17 / Th17 / ROR / 自己免疫疾患 / ダイオキシン / レポーターアッセイ |
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| Outline of Final Research Achievements |
The retinoic acid receptor-related orphan receptor (ROR) and aryl hydrocarbon receptor (AhR) are key regulators of helper T (Th)17 cell differentiation. However, it remains unclear whether environmental chemicals, including dioxins, affect Th17 cell functions. In this study, we examined the AhR activity of 200 environmental chemicals using DR-EcoScreen assay, and found that several pesticides and isoflavones enhanced AhR-mediated transcriptional activity. These compounds increased the expression of IL-17A mRNA without effecting ROR mRNA levels in mouse T lymphoma EL4 cells. On the other hand, these chemicals did not affect the IL-17A gene expression in ROR- or AhR-knockdown EL4 cells. Taken together, these results suggest that several AhR agonists might enhance IL-17 production via ROR and AhR. This study also provides the evidence that environmental chemicals can act as modulators of IL-17 gene expression in immune cells.
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] Simple and rapid determination of PCDD/Fs in flue gases from various waste incinerators in China using DR-EcoScreen cells.2014
Author(s)
Zhou Z, Zhao B, Kojima H, Takeuchi S, Takagi Y, Tateishi N, Iida M, Shiozaki T, Xu P, Qi L, Ren Y, Li N, Zheng S, Zhao H, Fan S, Zhang T, Liu A, Huang Y.
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Journal Title
Chemosphere
Volume: 102
Pages: 24-30
DOI
Related Report
Peer Reviewed
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[Journal Article] Tyk2 is a therapeutic target for psoriasis-like skin inflammation.2014
Author(s)
Ishizaki M, Muromoto R, Akimoto T, Sekine Y, Kon S, Diwan M, Maeda, H, Togi S, Shimoda K, Oritani K, Matsuda T.
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Journal Title
Int. Immunol.
Volume: 26
Issue: 5
Pages: 257-267
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] Comparative study on human and mouse nuclear receptor activities of hepatomegaly-inducing perfluorooctanoic acid and its related compounds.2014
Author(s)
Hiroyuki Kojima, Shinji Takeuchi, Fumihiro Sata, Hiroyuki Nakajima, Chise Noomote, Chieri Fujino, Naoto Uramaru, Shigeyuki Kitamura, Koichi Yoshinari
Organizer
International Conference of Asian Environmental Chemistry (ICAEC2014)
Place of Presentation
Convention Center, Chulabhorn Research Institute (Bangkok, Thailand)
Year and Date
2014-11-24 – 2014-11-26
Related Report
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[Presentation] Differential determination of plasticizers and organophosphorus flame retardants in residential indoor air.2014
Author(s)
Shinji Takeuchi, Kazuo Jin, Masayuki Sato, Satoshi Kobayashi, Hiroyuki Kojima, Ikue Saito, Hidenori Uemura, Toshiko Tanaka-Kagawa, Hideto Jinno
Organizer
International Conference of Asian Environmental Chemistry (ICAEC2014)
Place of Presentation
Convention Center, Chulabhorn Research Institute (Bangkok, Thailand)
Year and Date
2014-11-24 – 2014-11-26
Related Report
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