| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In this study, separate two-step real-time PCR assays were used to detect 14 RNA viruses and a DNA virus. 1) 512 nasal aspirates from the pediatric hospital with respiratory symptoms and 2) 286 gargle specimens (obtained by rinsing the throat with distilled water) from 8 children once a week were tested. 1) Of 512 specimens analyzed, 424 (83%) were positive for 1 or more viruses. Human rhinovirus species A (HRV-A) was the most frequently detected (22% of total samples). 2) The parents of children noted the existence of respiratory symptoms and systemic symptoms (fever or rash) at the time of each sampling. HRV-A was the most common agents (5%) detected from the samples of children without any symptoms. These results suggested that HRV-A is highly common respiratory agent in children and the clinical severity of HRV infections is dependent on mainly host’s factors. Respiratory viral infection without any symptoms may play an important role in the viral circulation in human populations.
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