Analysis of relationship between genotypes and inhabit environment of Legionella spp. detected by amoebic co-culture method
| Project/Area Number |
24590841
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Public health/Health science
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| Research Institution | Osaka Prefectural Institute of Public Health |
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Principal Investigator |
EDAGAWA AKIKO 大阪府立公衆衛生研究所, 衛生化学部, 主任研究員 (80321941)
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| Co-Investigator(Kenkyū-buntansha) |
木村 明生 大阪府立公衆衛生研究所, 企画総務部, 課長 (00250283)
河原 隆二 大阪府立公衆衛生研究所, 感染症部, 主任研究員 (10332454)
宮本 比呂志 佐賀大学, 医学部, 教授 (40229894)
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| Project Period (FY) |
2012-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | レジオネラ / アカントアメーバ / アメーバ共培養法 / co-culture / 培養不能菌 / VBNC / LLAP / SBT / アメーバ / 環境水 / 浴槽水 / L.pneumophila / 培養不能 |
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| Outline of Final Research Achievements |
In this study we performed amoebic co-culture method to investigate legionella contamination of environmental water samples. Of 120 water samples, 75.1% (91 samples) were positive for Legionella spp. by amoebic co-culture method combined with real-time PCR method. we performed Sequence-Based Typing to analyze relationship between genotypes and inhibit environment of L. pneumophila detected by amoebic co-culture method and mip gene PCR, however we could not obtain positive data. Among the 91 positive samples, 17 samples (14.2%) showed over 10% increase of bacteria numbers. Following sequence analysis found LLAP type such as L. lytica from the positive samples. Our results suggested that Legionella spp. including viable but non-culturable form highly inhabited environmental water.
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Report
(6 results)
Research Products
(7 results)
