• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of relationship between genotypes and inhabit environment of Legionella spp. detected by amoebic co-culture method

Research Project

Project/Area Number 24590841
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Public health/Health science
Research InstitutionOsaka Prefectural Institute of Public Health

Principal Investigator

EDAGAWA AKIKO  大阪府立公衆衛生研究所, 衛生化学部, 主任研究員 (80321941)

Co-Investigator(Kenkyū-buntansha) 木村 明生  大阪府立公衆衛生研究所, 企画総務部, 課長 (00250283)
河原 隆二  大阪府立公衆衛生研究所, 感染症部, 主任研究員 (10332454)
宮本 比呂志  佐賀大学, 医学部, 教授 (40229894)
Project Period (FY) 2012-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsレジオネラ / アカントアメーバ / アメーバ共培養法 / co-culture / 培養不能菌 / VBNC / LLAP / SBT / アメーバ / 環境水 / 浴槽水 / L.pneumophila / 培養不能
Outline of Final Research Achievements

In this study we performed amoebic co-culture method to investigate legionella contamination of environmental water samples. Of 120 water samples, 75.1% (91 samples) were positive for Legionella spp. by amoebic co-culture method combined with real-time PCR method. we performed Sequence-Based Typing to analyze relationship between genotypes and inhibit environment of L. pneumophila detected by amoebic co-culture method and mip gene PCR, however we could not obtain positive data. Among the 91 positive samples, 17 samples (14.2%) showed over 10% increase of bacteria numbers. Following sequence analysis found LLAP type such as L. lytica from the positive samples. Our results suggested that Legionella spp. including viable but non-culturable form highly inhabited environmental water.

Report

(6 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (7 results)

All 2016 2015 2014 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results)

  • [Journal Article] アメーバ共培養-LAMP法を用いた水景施設におけるレジオネラ属菌生息調査2016

    • Author(s)
      1.枝川亜希子、木村明生、足立伸一、松島加代、宮本比呂志
    • Journal Title

      日本防菌防黴学会誌

      Volume: 44 Pages: 585-589

    • NAID

      40021020835

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Investigation of Legionella Contamination in Bath Water Samples by Culture, Amoebic Co-Culture, and Real-Time Quantitative PCR Methods.2015

    • Author(s)
      Edagawa A, Kimura A, Kawabuchi-Kurata T, Adachi S, Furuhata K, Miyamoto H
    • Journal Title

      Int J Environ Res Public Health.

      Volume: 12(10) Pages: 13118-30

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] アメーバ共培養-LAMP法を用いた水景施設レジオネラ属菌生息調査2016

    • Author(s)
      枝川亜希子、木村明生、足立伸一、宮本比呂志
    • Organizer
      第43回日本防菌防黴学会年次大会
    • Place of Presentation
      東京
    • Year and Date
      2016-09-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] 培養法、アメーバ共培養法、およびリアルタイムPCR法を用いた浴槽水のレジオネラ汚染調査2016

    • Author(s)
      枝川亜希子
    • Organizer
      第28回臨床微生物迅速診断研究会総会
    • Place of Presentation
      福岡
    • Related Report
      2016 Annual Research Report
  • [Presentation] アメーバ共培養法を用いた浴槽水中に存在するレジオネラ属菌汚染実態の解明2015

    • Author(s)
      1.枝川亜希子、木村明生、田中榮次、足立伸一、宮本比呂志
    • Organizer
      日本防菌防黴学会
    • Place of Presentation
      大阪
    • Year and Date
      2015-09-01
    • Related Report
      2015 Research-status Report
  • [Presentation] レジオネラ検査ろ過濃縮法における メンブランフィルター材質の回収率比較2014

    • Author(s)
      枝川亜希子, 木村明生, 三輪由佳, 田中榮次, 足立伸一, 宮本比呂志
    • Organizer
      日本防菌防黴学会
    • Place of Presentation
      東京
    • Year and Date
      2014-09-24 – 2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] レジオネラ属菌のアメーバ内増殖能を利用したアメーバ共培養法の検討2012

    • Author(s)
      枝川亜希子、木村明生、田中榮次、足立伸一、宮本比呂志
    • Organizer
      第39回日本防菌防黴学会年次大会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi