| Project/Area Number |
24590843
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Public health/Health science
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| Research Institution | Osaka Prefectural Institute of Public Health |
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Principal Investigator |
TAKAHASHI KAZUO 大阪府立公衆衛生研究所, その他部局等, その他 (10171472)
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| Co-Investigator(Kenkyū-buntansha) |
SAKON Naomi 大阪府立公衆衛生研究所, 感染症部ウイルス課, 主任研究員 (50291216)
KURATA Takako 大阪府立公衆衛生研究所, 感染症部ウイルス課, 主任研究員 (70435890)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 起因微生物 / 迅速 / PCR法 / 網羅的 / Qプローブ法 / 感染症 / 病原微生物 / 高感度 / 病原体 / 迅速診断法 / 薬剤耐性 |
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| Outline of Final Research Achievements |
We developed more rapid(within 1 hour including DNA extraction), highly sensitive,multipathogen-targetted detection methods (referred to Q probe method) against representative pathogens of infectious diseases. The Q-probe method for pertussis revealed almost equivalent quality with more rapid performance compared to a conventional LAMP (Loop-Mediated Isothermal Amplification)method. The Q-probe method for mycoplasma infection revealed that 72% of M.pneumoniae was defined within 1 h to be resistant to macrolide type of antibiotics, suggesting that this more rapid method confers shortening the treatment period. The Q-probe method for campylobacter also revealed its superior quality with more rapid performance compared to a conventional culture isolation method.
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