Can we get new insights into epidemiology of tuberculosis through whole genome sequencing analysis ?

• Project/Area Number: 24590845
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Public health/Health science
• Research Institution: Kobe Institute of Health
• Principal Investigator: IWAMOTO Tomotada 神戸市環境保健研究所, その他部局等, 研究員 (70416402)
• Co-Investigator(Renkei-kenkyūsha): WADA Takayuki 長崎大学, 熱帯医学研究所, 助教 (70332450)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 結核 / 感染症 / ゲノム / 分子疫学 / 公衆衛生 / 細菌 / 次世代シーケンサー / ゲノム疫学 / SNVs / 遺伝型別 / VNTR / SNP

Outline of Final Research Achievements

Detection and cutting of transmission routes of Mycobacterium tuberculosis is one of the most important public health actions to control the expansion of tuberculosis.Whole-genome sequencing (WGS) gives greater discrimination than widely used genotyping methods, which should improve the ability to trace a transmission of M. tuberculosis.In this study, we focused on one genotype strains (30 strains) to verify the effectiveness of WGS to unvail the transmission routes of the genotype strains. Our WGS analysis idenitified in total 35 single nuclotide variants (SNVs) which subdivided the 30 strains into highly probable transmission chains.This means that current molecular epidemiological study complement with WGS could be a practical approach to get deeper traceability of transmission of M. tuberculosis.

Research Products

• [Journal Article] Predominant Mycobacterium tuberculosis families and high rate of transmission among new cases are not associated with primary multidrug resistance in Lima. (2015)
  • Author(s): Barletta F, Otero L, de Jong B, Iwamoto T, Arikawa K, Van der Stuyft P, Niemann S, Merker M, Uwizeye C, Seas C, and Rigouts L.
  • Journal Title: J. Clin. Microbiol. Volume: 53 Issue: 6 Pages: 1854-1863
  • DOI: 10.1128/jcm.03585-14
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The association between Mycobacterium tuberculosis genotype and drug resistance in Peru. (2015)
  • Author(s): Grandjean L, Iwamoto T, Lithgow A, Gilman R.H., Arikawa K, Nakanishi N, Martin L, Castillo E, Alarcon V, Coronel J, Solano W, Aminian M, Guezala C, Rastogi N, Couvin D, Sheen P, Zimic M, Moore D
  • Journal Title: PLoS ONE Volume: 10 Issue: 5 Pages: e0126271-e0126271
  • DOI: 10.1371/journal.pone.0126271
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ntra-subspecies sequence variability of the MACPPE12 gene in Mycobacterium avium subsp. Hominissuis (2014)
  • Author(s): Iwamoto T, Arikawa K, Nakajima C, Nakanishi N, Nishiuchi Y, Yoshida S, Tamaru A,Tamura Y, Hoshino Y, Yoo H, Park YK, Saito H, Suzuki Y
  • Journal Title: Infect. Genet. Evolu. Volume: 21 Pages: 476-483
  • DOI: 10.1016/j.meegid.2013.08.010
  • Peer Reviewed / Open Access
• [Journal Article] 36) 複数自治体をまたぐ広域的結核分子疫学の基盤構築：JATA (12)-VNTR型別に基づくクラスター形成とその傾向． (2013)
  • Author(s): 和田崇之，田丸亜貴，岩本朋忠，有川健太郎，中西典子，小向潤，松本健二，長谷篤
  • Journal Title: 結核 Volume: 88 Pages: 393-398
  • Peer Reviewed
• [Journal Article] 37) JATA (12)-VNTR型別による結核集団発生事例の菌株異同調査 (2013)
  • Author(s): 田丸亜貴，和田崇之，岩本朋忠，長谷篤
  • Journal Title: 結核 Volume: 88 Pages: 399-403
  • Peer Reviewed
• [Journal Article] 38) Mycobacterium fortuitumを対象としたZiehl-Neelsen染色法と蛍光染色法における抗酸性の比較検討 (2013)
  • Author(s): 吉田志緒美，露口一成，鈴木克洋，冨田元久，岡田全司，林清二，有川健太郎，岩本朋忠
  • Journal Title: 結核 Volume: 88 Pages: 461-467
  • Peer Reviewed
• [Journal Article] 51) 結核分子疫学による疫学動向の推察 (2013)
  • Author(s): 岩本朋忠
  • Journal Title: 結核 Volume: 88 Pages: 331-335
• [Journal Article] 23) Evolutionary robust SNPs reveal the misclassification of Mycobacterium tuberculosis Beijing family strains into sublineages. (2013)
  • Author(s): Noriko Nakanishi, Takayuki Wada, Kentaro Arikawa, Julie Millet, Nalin Rastogi, and Tomotada Iwamoto
  • Journal Title: Infect. Genet. Evol. Volume: 16 Pages: 174-177
  • DOI: 10.1016/j.meegid.2013.02.007
  • Peer Reviewed
• [Journal Article] 22) Genetic diversity and transmission characteristics of Beijing family strains of Mycobacterium tuberculosis in Peru. (2012)
  • Author(s): T. Iwamoto, L. Grandjean, K. Arikawa, N. Nakanishi, L. Caviedes, J. Coronel, P. Sheen, T. Wada, C. A. Taype, M.A. Shaw, D>A.J. Moore, and R.H. Gilman.
  • Journal Title: PLoS ONE Volume: 7 Issue: 11 Pages: e49651-e49651
  • DOI: 10.1371/journal.pone.0049651
  • Peer Reviewed
• [Presentation] 多剤耐性結核菌の二次変異保有状況の解析 (2015)
  • Author(s): 有川健太郎，吉田志緒美，岩本朋忠
  • Organizer: 第88回 日本細菌学会総会
  • Place of Presentation: 岐阜
  • Year and Date: 2015-03-28
• [Presentation] 都市型結核分子疫学へのゲノム解析の活用 (2015)
  • Author(s): 岩本朋忠
  • Organizer: 第19回 国際結核セミナー
  • Place of Presentation: 東京
  • Year and Date: 2015-03-05
• [Presentation] 結核菌分子疫学による神戸市内蔓延株の網羅的解析ならびに外国人結核との比較 (2014)
  • Author(s): 有川健太郎，中西典子，岩本朋忠，藤山理世，松林恵介，水尻節子，白井千香，伊地智昭浩
  • Organizer: 第89回 日本結核病学会総会
  • Place of Presentation: 岐阜
  • Year and Date: 2014-05-10
• [Presentation] ワクチン接種副反応患者の生体内で起こったBCG Tokyo 172株の微小進化 (2014)
  • Author(s): 岩本朋忠、有川健太郎、中西典子、瀧井猛将
  • Organizer: 第87回 日本細菌学会総会
  • Place of Presentation: 東京
• [Presentation] 結核菌分子疫学による地域内蔓延株の網羅的解析ならびに外国人結核との比較 (2014)
  • Author(s): 有川健太郎、中西典子、岩本朋忠
  • Organizer: 第87回 日本細菌学会総会
  • Place of Presentation: 東京
• [Presentation] 結核分子疫学への新技術「次世代シーケンサー」活用の基礎的検討 (2013)
  • Author(s): 岩本朋忠、有川健太郎、中西典子、藤山理世、松林恵介、山下真理子、水尻節子、白井千香、伊地智昭浩
  • Organizer: 第111回日本結核病学会 近畿地方会
  • Place of Presentation: 大阪
• [Presentation] 神戸市における外国人結核の分子疫学 (2013)
  • Author(s): 有川健太郎、中西典子、岩本朋忠、藤山理世、松林恵介、山下真理子、水尻節子、白井千香、伊地智昭浩
  • Organizer: 第111回日本結核病学会 近畿地方会
  • Place of Presentation: 大阪
• [Presentation] BCGワクチン接種副反応事例から得たBCG Tokyo 172-1株に生じた遺伝子変異の検出 (2013)
  • Author(s): 岩本朋忠、有川健太郎、吉田志緒美、露口一成、鈴木克洋
  • Organizer: 第44回 結核・非定型抗酸菌症治療研究会
  • Place of Presentation: 東京
• [Presentation] Genomic Diversity of Mycobacterium avium subsp. hominissuis Provides a Perspective on its Host Tropism (2013)
  • Author(s): Tomotada Iwamoto, Chie Nakajima, Yasuhiko Suzuki
  • Organizer: US-JAPAN COOPERATIVE MEDICAL SCIENCE PROGRAM
  • Place of Presentation: 札幌
• [Presentation] 結核菌北京型1,054株を用いた遺伝的系統解析法の評価 (2013)
  • Author(s): 中西典子、和田崇之、有川健太郎、岩本朋忠
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉
• [Presentation] Genetic diversity of Beijing family strains of Mycobacterium tuberculosis in Peru compared with that of strains from East Asia (2012)
  • Author(s): T. Iwamoto, L. Grandjean, K. Arikawa, N. Nakanishi, J. Coronel, L. Caviedes, P. Sheen, T. Wada, C. Taype, MA. Shaw, DAJ Moore, RH Gilman.
  • Organizer: 11 th International Conference on Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases
  • Place of Presentation: New Orleans, USA