| Project/Area Number |
24590847
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
ASARI Masaru 旭川医科大学, 医学部, 准教授 (40360979)
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| Co-Investigator(Kenkyū-buntansha) |
OMURA Tomohiro 京都大学, 医学(系)研究科(研究院), 助教 (00430935)
OKA Kumiko 旭川医科大学, 医学部, 助教 (60571891)
大村 友博 京都大学, 医学(系)研究科(研究院), 助教 (00439035)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 個人識別 / 挿入欠失多型 / 人工核酸LNA / ユニバーサルプライマー / 人工核酸ＬＮＡ / PCR / 蛍光標識 |
|---|
| Outline of Final Research Achievements |
We analyzed the effects of locked nucleic acids (LNAs) for the efficiency of fluorescent labeling of PCR products. When the 24-base universal sequence with a higher Tm value was used, the introduction of a few LNAs significantly improved the efficiency, compared to the results from the 18-base universal sequence. We also selected 37 autosomal insertion/deletion (Indel) polymorphisms, and calculated the frequencies of alleles from 100 Japanese individuals. The matching probability was 2×10(-15), and the combination of selected Indels would be useful for individual discrimination. In multiplex Indel genotyping, fluorescent universal primers with LNAs produced higher signals than non-LNA fluorescent universal primers, and the increasing effects were identified especially in the low concentration (0.1 μM) of these primers.
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