Development of a novel DNA typing method using 37 STR loci
| Project/Area Number |
24590854
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
OZEKI Munetaka 京都大学, 医学(系)研究科(研究院), 助教 (80549618)
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| Co-Investigator(Renkei-kenkyūsha) |
TAMAKI Keiji 京都大学, 医学研究科, 教授 (90217175)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | DNA鑑定 / アレル頻度 / 法医学 |
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| Outline of Final Research Achievements |
Allele frequencies for 37 short tandem repeat (STR) autosomal loci were obtained from a sample of 175 unrelated individuals in a Japanese population. Novel variant alleles were observed and defined by its sequence on D5S818, D19S433, F13B and FGA. Linkage disequilibrium was observed between FESFPS and Penta E on chromosome 15. To aid DNA identification using 36 STR loci for kinship analysis, likelihood ratio distributions were estimated. However, a typing kit targeting 36 STR loci is not suitable for the analysis of damaged DNA. In this study, we developed a novel miniplex DNA identification system targeting 7 STR loci (D3S1744, D5S818, D8S1179, D10S2325, Penta D, Penta E, and vWA) that was optimized for use in combination with MiniFiler for the Japanese population.
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Report
(4 results)
Research Products
(3 results)
