Role of tissue heme protein on the toxicokinetics of carbon monoxide.

• Project/Area Number: 24590856
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Kagawa University
• Principal Investigator: KINOSHITA HIROSHI 香川大学, 医学部, 教授 (00284357)
• Co-Investigator(Kenkyū-buntansha): AMENO Kiyoshi 香川大学, 医学部, 准教授 (50019626) ; TANAKA Naoko 香川大学, 医学部, 助教 (60700052)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 一酸化炭素 / 中毒 / センサーガスクロマトグラフィー / ヘモグロビン / 飽和度 / 体内動態 / 血液 / 筋肉 / 組織 / 死後拡散 / 毒性 / ミオグロビン

Outline of Final Research Achievements

We have applied the sensor gas chromatography for the measurement of carboxyhemoglobin (CO-Hb) saturation in blood. It is highly sensitive for carbon monoxide, and it may be applicable for other forensic samples.
No remarkable difference of CO-Hb saturation was observed between heart blood and peripheral venous blood in carbon monoxide related cases. And there was no effect of sample dilution on the value of CO-Hb in case of concentrated blood.

Research Products

• [Journal Article] Application of sensor gas chromatography for the determination of carbon monoxide in forensic medicine. (2014)
  • Author(s): Tanaka N, Kinoshita H, Takakura A, Jamal M, Kumihashi M, Uchiyama Y, Tsutsui K, Ameno K.
  • Journal Title: Current study of Environmental and Medical Sciences Volume: 7 Pages: 9-11
  • Peer Reviewed
• [Journal Article] オキシメータ エーボックス4000を用いた一酸化炭素ヘモグロビン飽和度測定における試料希釈の影響 (2014)
  • Author(s): 田中直子、飴野 清、モストファジャーマル、組橋 充、宮武伸行、木下博之
  • Journal Title: 法医病理 Volume: 20 Pages: 39-40
  • Peer Reviewed
• [Presentation] センサーガスクロマトグラフによる一酸化炭素濃度測定 (2013)
  • Author(s): 田中直子、飴野清、jamal M、組橋充、木下博之
  • Organizer: 日本法医学会学術全国集会
  • Place of Presentation: 札幌
• [Book] Evaluation of carboxyhemoglobin (CO-Hb) by oximeter in forensic practice. In "Carbon monoxide: Sources, uses and hazards ", (2012)
  • Author(s): Kinoshita H, Tanaka N, Jamal M, Kumihashi M, Ameno K.
  • Publisher: Nova Science Publishers Inc,