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Development of automatic plankton test for diagnosis of drowning

Research Project

Project/Area Number 24590861
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Legal medicine
Research InstitutionKagoshima University

Principal Investigator

AGO Kazutoshi  鹿児島大学, 医歯(薬)学総合研究科, 准教授 (20102056)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Keywords法医病理 / 溺死 / プランクトン検査 / 珪藻検査 / 壊機法 / 画像解析 / 自動化 / 法医解剖
Outline of Final Research Achievements

Two automatic systems: acid digestion of samples and detection of plankton, are necessary for automatic plankton (diatom) test which diagnoses death by drowning. In this study, the potential results were acquired on the automatic detection of plankton. The automatic system is composed of image tiling software (e-Tiling, MITANI Corpolation), image analysis software (WinROOF, MITANI Corpolation) and light microscopy which is equipped with motorized stage (ProScan, PRIOR Scientific). Though the time of tiling by e-Tiling was too long (over 1 hour /a slide), there was a merit where the information of prepared slides is permanently preserved as digital image. On WinROOF, though the ability of discrimination of plankton species was not sufficient, the speed of search was remarkably faster than manual detection. It was revealed that the new system adopting e-Tiling and WinROOF would bring an improvement to the plankton test for diagnosis of drowning.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (2 results)

  • [Journal Article] Postmortem wounds caused by cookie-cutter sharks (Isistius species): an autopsy case of a drowning victim2015

    • Author(s)
      Takahito Hayashi, Eri Higo, Hideki Orito, Kazutoshi Ago, Mamoru Ogata
    • Journal Title

      Forensic Science, Medicine, and Pathology

      Volume: 11 Issue: 1 Pages: 119-121

    • DOI

      10.1007/s12024-014-9597-9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ダルマザメの咬傷と思われる類円形あるいは類楕円形の損傷を認めた溺死体の1剖検例2013

    • Author(s)
      吉野直明,林 敬人,吾郷一利,吾郷美保子,小片 守
    • Journal Title

      法医学の実際と研究

      Volume: 56 Pages: 193-197

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 2011年における鹿児島県の浴室内突然死例の検討-死後CT検査導入による検案時の死因判定の変化について-2013

    • Author(s)
      吾郷 一利,林 敬人,吾郷 美保子,原田 朋幸,小片 守
    • Journal Title

      鹿児島大学医学雑誌

      Volume: 65 Pages: 1-8

    • NAID

      120005447094

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 画像連結ならびに画像解析ソフトウエアを用いたプランクトン検索自動化の試み2015

    • Author(s)
      吾郷一利,林 敬人,中前琢磨,小片 守
    • Organizer
      第99次日本法医学会学術全国集会
    • Place of Presentation
      高知市文化プラザ かるぽーと(高知県,高知市)
    • Year and Date
      2015-06-11 – 2015-06-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 溺死診断のためのプランクトン検査自動化の試み-画像解析による形動検索2014

    • Author(s)
      吾郷一利,隈元健吾,桑山紗也華,田代侑馬,田村誠也,原口めぐみ,林 敬人,小片 守
    • Organizer
      第64回日本法医学会学術九州地方集会
    • Place of Presentation
      鹿児島県市町村自治会館(鹿児島県,鹿児島市)
    • Year and Date
      2014-10-10 – 2014-10-11
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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