| Project/Area Number |
24590863
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
NOSAKA Mizuho 和歌山県立医科大学, 医学部, 助教 (00244731)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIDA Yuko 和歌山県立医科大学, 医学部, 講師 (10364077)
KIMURA Akihiko 和歌山県立医科大学, 医学部, 准教授 (60136611)
KONDO Toshikazu 和歌山県立医科大学, 医学部, 教授 (70251923)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 深部静脈血栓塞栓症 / 血管内皮前駆細胞 / サイトカイン / 血栓陳旧度 / MMP / プラスミノーゲン活性因子 / IFN-gamma / TNF-alpha / IL-6 / TNF-Rp55 / 血管新生 / EPC |
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| Outline of Final Research Achievements |
Deep vein thrombosis (DVT) is multifactorial and often results from a combination of risk factors such as obesity, pregnancy, aging and malignancy. On the GFP chimeric mice, the GFP-positive cells were detected in their thrombi. This phenomenon was a proof of the myeloid cells migrated from their bone marrow. Eight-week-old male wild type mice were employed in this study. DVT was induced by the ligation of inferior vena cava. At 1, 3, 5, 7, 10, 14 and 21 days, histological and immunohistochemical analyses were performed. In a thrombus age of less than 3 days, CD34+/Flk-2+ EPC were not detected. The intrathrombotic EPC were initially observed in thrombus aged 5 days, and their number increased with advances in thrombus age. Our observations indicate the participation of EPC in the thrombi inducing the accumulation of extracellular matrix components, and therefore, detection of EPC could be a useful marker for thrombus age determination.
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