Development of analytical methods for the determination of drugs and poisons with molecularly imprinted polymers and its forensic application

• Project/Area Number: 24590865
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Seirei Christopher University (2016); Showa University (2012-2015)
• Principal Investigator: KUMAZAWA Takeshi 聖隷クリストファー大学, 看護学部, 教授 (00186470)
• Co-Investigator(Kenkyū-buntansha): 長谷川 智華 東邦大学, 医学部, 助教 (10468689) ; 李 暁鵬 (中内暁博) 昭和大学, 医学部, 准教授 (90245829)
• Project Period (FY): 2012-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 固相抽出 / 分子インプリントポリマー / 薬毒物分析 / 分離分析法 / 薬物 / 農薬 / 固相抽出法 / 毒物

Outline of Final Research Achievements

In the present study, we examined the determination of drugs and poisons in human body fluids using molecularly imprinted solid-phase extraction (MISPE) as highly selective sample clean-up technique. After MISPE, the analytes extracted from whole blood, plasma, and urine were quantified using gas chromatography (GC), high performance liquid chromatography (HPLC) , GC-mass spectrometry (MS), HPLC-MS. The results showed that drugs and poisons can be efficiently extracted from crude biological samples by MISPE with relatively good reproducibility. The present analytical method will be a useful for the screening and quantitative determination of xenobiotics, in clinical and forensic analysis.

Research Products

• [Journal Article] Rapid and highly sensitive analysis of benzodiazepines and tandospirone in human plasma by automated on-line column-switching UFLC-MS/MS. (2017)
  • Author(s): Lee, X.-P., Shouji, Y., Kumazawa, T., Hasegawa, C., Fujishiro, M., Sato, J., Hasegawa, I., Sato, K.
  • Journal Title: Legal Medicine Volume: 24 Pages: 36-55
  • DOI: 10.1016/j.legalmed.2016.11.005
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] High-throughput determination of nonsteroidal anti-inflammatory drugs in human plasma by HILIC-MS/MS (2014)
  • Author(s): Nemoto T, Lee X-P, Kumazawa T, Hasegawa C, Fujishiro M, Marumo A, Shouji Y, Inagaki K, Sato K
  • Journal Title: J Pharm Biomed Anal Volume: 88 Pages: 71-80
  • DOI: 10.1016/j.jpba.2013.08.023
  • Peer Reviewed
• [Journal Article] Spin tip solid-phase extraction and HILIC-MS-MS for quantitative determination of methamphetamine and amphetamine in human plasma (2014)
  • Author(s): Marumo A, Kumazawa T, Lee X-P, Hasegawa C, Sato K
  • Journal Title: J Liq Chrom Relat Tech Volume: 37 Issue: 3 Pages: 420-432
  • DOI: 10.1080/10826076.2012.745145
  • Peer Reviewed
• [Journal Article] High-throughput determination of barbiturates in human plasma using on-line column-switching ultra-fast liquid chromatography-tandem mass spectrometry (2013)
  • Author(s): Lee X-P, Kumazawa T, Hasegawa C, Arinobu T, Seno H, Suzuki O, Sato K
  • Journal Title: Forensic Toxicol Volume: 31 Issue: 1 Pages: 9-20
  • DOI: 10.1007/s11419-012-0155-4
  • Peer Reviewed
• [Journal Article] Molecularly imprinted solid-phase extraction for the selective determination of methamphetamine, amphetamine, and methylenedioxyphenylalkylamine designer drugs in human whole blood by gas chromatography-mass spectrometry (2012)
  • Author(s): Takeshi Kumazawa
  • Journal Title: Journal of Separational Sciences Volume: 35 Issue: 5-6 Pages: 726-733
  • DOI: 10.1002/jssc.201100924
  • Peer Reviewed
• [Journal Article] A simple and reliable method for quantifying plasma concentrations of tetracyclic antidepressants using monolithic silica solid-phase extraction tips (2012)
  • Author(s): Hayashi D, Kumazawa T, Hasegawa C, Lee X-P, Marumo A, Uchigasaki S, Kawamura M, Sato K
  • Journal Title: Forensic Toxicol Volume: 30 Issue: 2 Pages: 98-105
  • DOI: 10.1007/s11419-012-0132-y
  • Peer Reviewed
• [Journal Article] A new method for quantitative determination of dimemorfan in human plasma using monolithic silica solid-phase extraction tips (2012)
  • Author(s): Hasegawa C, Kumazawa T, Terada M, Lee X-P, Sato K, Uchigasaki S, Kurosaki K
  • Journal Title: Legal Med Volume: 14 Issue: 5 Pages: 267-271
  • DOI: 10.1016/j.legalmed.2012.03.004
  • Peer Reviewed
• [Presentation] High-throughput determination of barbiturates in human plasma using on-line column-switching ultra fast liquid chromatography-tandem mass spectrometry. (2012)
  • Author(s): Lee X.-P.
  • Organizer: The 50th Annual Meeting of the International Association of Forensic Toxicologists (TIAFT)
  • Place of Presentation: 浜松
• [Book] The neuropathology of drug addictions and substance misuse. (2016)
  • Author(s): Takeshi Kumazawa
  • Total Pages: 1182
  • Publisher: Elsevier