Study of the individual identity from the sample which used for the forensic detection by direct PCR method
| Project/Area Number |
24590867
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Nihon University |
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Principal Investigator |
TIE Jian 日本大学, 医学部, 専任講師 (40277439)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 法科学試料 / 予備検査 / DNA多型 / 直接PCR増幅 / 個人識別 / 法医学 / 予備試験 / 直接PCR / STR / ヒト体液斑 / ヒト爪 / STR多型 / ヒト血痕 / 人血確認実験 / 実験後残存糸 |
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| Outline of Final Research Achievements |
A purpose of this study is to detect DNA polymorphism using the direct PCR method from a forensic sample or a sample which used for forensic medicine pretest. The normal forensic sample includes a bloodstain, saliva stain and sperm stain, as a matter of common knowledge, there are the most bloodstains. A qualitative examination is necessary before detecting DNA polymorphisms. However, it cannot •secure enough amount sample for the next DNA analysis when use up a sample in preliminary examination. Therefore in this study just examined a DNA analysis by reuse in the sample which used in preliminary examination. The PCR of 15 STR polymorphisms were amplified by a sample after the qualitative inspection of a bloodstains, saliva stains and the sperm stains, and the genotypes were able to detect from most samples.
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Report
(4 results)
Research Products
(3 results)
