| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
A purpose of this study is to detect DNA polymorphism using the direct PCR method from a forensic sample or a sample which used for forensic medicine pretest. The normal forensic sample includes a bloodstain, saliva stain and sperm stain, as a matter of common knowledge, there are the most bloodstains. A qualitative examination is necessary before detecting DNA polymorphisms. However, it cannot •secure enough amount sample for the next DNA analysis when use up a sample in preliminary examination. Therefore in this study just examined a DNA analysis by reuse in the sample which used in preliminary examination. The PCR of 15 STR polymorphisms were amplified by a sample after the qualitative inspection of a bloodstains, saliva stains and the sperm stains, and the genotypes were able to detect from most samples.
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