Development of high-sensitive and fast drug screening systems in body materials for investigation on cause of death

• Project/Area Number: 24590872
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Fukuoka University
• Principal Investigator: HARA Kenji 福岡大学, 医学部, 講師 (00090738)
• Co-Investigator(Kenkyū-buntansha): WATERS Brian 福岡大学, 医学部, 助教 (00609480) ; KUBO Shin-ichi 福岡大学, 医学部, 教授 (10205122) ; KASHIWAGI Masayuki 福岡大学, 医学部, 准教授 (70301687) ; MATSUSUE Aya 福岡大学, 医学部, 講師 (70309920)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 法医中毒学 / 体組織試料 / 試料処理 / 薬物スクリーニング / GC-MS / LC-MS/MS / 迅速 / 簡素化 / 薬毒物分析 / 質量分析法 / ガスクロマトグラフィー / 高速液体クロマトグラフィー / 剖検試料 / 高速測定 / 体組織中薬物分析 / 試料処理の簡素化 / アセトニトリル抽出 / イオン交換抽出カラム / GC-MS測定の高速化 / 水素キャリアガス / 一定流量 / GC-MSの高速化

Outline of Final Research Achievements

In the investigation of the cause of death in forensic toxicology, analysis of the drug is important. We have examined the development of screening methods for searching for a wide variety of drugs. Postmortem samples, since often contains a large amount of lipids, removal of lipids were mainly studied. Preparation of postmortem sample was studied on ion pair acetonitrile extraction, lipid removal by a specific filter and by organic solvent washing, and ion exchange purification. Fast GC-MS by use of two connected column, was able to apply to the measurement of rapid and sensitive. Except highly denatured sample, the methods developed in this study were feasible for practical applications. In this sample preparation, a wide range of from lipophilic drugs to hydrophilic drugs can be measured by a combination of GC-MS and LC-MS / MS.

Research Products

• [Journal Article] Autopsy report for a caffeine intoxication case and review of the current literature (2015)
  • Author(s): Yamamoto,T., Yoshizawa, K., Kubo, S., Emoto, Y., Hara, K., Waters, B., Umehara, T., Murase, T., Ikematsu, K.
  • Journal Title: J Toxicol Pathol Volume: 28 Pages: 33-36
  • NAID: 130004704151
  • Peer Reviewed
• [Journal Article] 食道・胃破裂を認めた解熱鎮痛剤中毒死の1剖検例 (2014)
  • Author(s): 高山 みお、Brian Waters、原 健二、松末 綾、柏木 正之、久保 真一
  • Journal Title: 法医学の実際と研究 Volume: 57 Pages: 29-34
  • Peer Reviewed
• [Presentation] Comparison of hydrogen and helium as carrier gas in a fast GC-MS (2013)
  • Author(s): K. Hara, B. Waters, M. Kashiwagi, A. Matsusue, M. Takayama, S.- I. Kubo
  • Organizer: Jahrestagung der DGRM 2013 (ドイツ語圏法医学会2013年会）
  • Place of Presentation: Saarbrücken (ドイツ）
• [Presentation] 腐敗試料からの薬物スクリーニング法の検討－簡易検査キットによる予備検査について－ (2012)
  • Author(s): 原 健二
  • Organizer: 第62回日本法医学会学術九州地方会
  • Place of Presentation: 熊本大