The basic study of central respiratory disturbances caused by carbon dioxide poisoning

• Project/Area Number: 24590874
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: TANAKA Toshiko 産業医科大学, 医学部, 講師 (80141745)
• Co-Investigator(Kenkyū-buntansha): SATO Hiroaki 産業医科大学, 医学部, 教授 (50441845)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 二酸化炭素中毒 / 延髄 / 呼吸中枢 / mRNA / マイクロアレイ / リアルタイムPCR / 二酸化炭素 / 遺伝子発現 / MAPKシグナルパスウェイ / サイトカイン / 呼吸抑制 / 縫線核 / 青斑核 / 前初期遺伝子 / 海馬CA1 / 好酸性化 / 壊死 / 幼弱

Outline of Final Research Achievements

The pathological effects of carbon dioxide (CO2) inhalation were studied at the concentration of more than 10% between infant rats and adult rats. The survival time and the incidence of eosinophilic pyramidal cells in the hippocampus CA1 of infant rats at 30% CO2 became greater than those in adult rats. The mainstream of respiratory nervous system has been reported to lie in the middle ventral area of medulla oblongata. The mRNA expression in this area was examined by the use of microarray and real-time PCR in the next step. The expressions of genes correlated with norepinephrine and serotonin did not change significantly, but the expressions of some immediate-early genes which related to MAPK signaling pathway or cytokine pathway were enhanced significantly. These expressions were not increased by the hypoxia only. The respiratory disturbances might possibly relate to these expressions induced by CO2 at the concentration of more than 10%.

Research Products

• [Presentation] 高濃度二酸化炭素吸入による延髄の遺伝子発現の検討 (2015)
  • Author(s): 田中 敏子，笠井 謙多郎，佐藤 寛晃
  • Organizer: 第99次日本法医学会学術全国集会
  • Place of Presentation: 高知市文化プラザ かるぽーと「高知県・高知市」
  • Year and Date: 2015-06-11
• [Presentation] 高濃度二酸化炭素による海馬障害における加齢の影響
  • Author(s): 田中 敏子，笠井 謙多郎，佐藤 寛晃
  • Organizer: 第63回日本法医学会学術九州地方集会
  • Place of Presentation: 福岡市九州大学病院キャンパス