Epigenetic mechanism under chronic starvation stress

• Project/Area Number: 24590888
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: Kyushu University
• Principal Investigator: SHU Takakura 九州大学, 大学病院, 助教 (40532859)
• Co-Investigator(Kenkyū-buntansha): KAWAI Keisuke 九州大学, 大学病院, 講師 (80325521)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: マイクロRNA / 長期飢餓ストレス / 神経性食欲不振症 / エピジェネティック / 飢餓ストレス / エピジェネティクス / DNAメチル化異常

Outline of Final Research Achievements

Using mice, sufficient decrease of body weight was observed under 40% food restriction and mortality rate was almost 0 %. Also we made usual chronic stress mice using 10-days water avoidance stress test. We found several miRNAs whose expressions were altered under chronic starvation stress using miRNA microarray. We found chronic starvation stress specific miRNAs by realtime RT-PCR compared with RNAs from usual chronic stress mouse hippocampus. The target of the specific miRNA might be a protein which is important for the neurological development. We could estimate one end of mechanisms of miRNAs altered by epigenetical regulation. Those important findings were obtained from the point of view that the elucidation of the pathophysiological mechanisms under chronic starvation stress condition such as anorexia nervosa and the development of the biomarkers of such condition.

Research Products

• [Presentation] 9歳発症の若年性神経性食欲不振症患者に対する一治療例 (2014)
  • Author(s): 高倉 修
  • Organizer: 第18回日本摂食障害学会学術集会
  • Place of Presentation: グランキューブ大阪 大阪府大阪市
  • Year and Date: 2014-09-13 – 2014-09-14
• [Presentation] 神経性食欲不振症の栄養回復期における虫垂炎発症について (2014)
  • Author(s): 高倉 修
  • Organizer: 第55回 日本心身医学会総会
  • Place of Presentation: 幕張メッセ 千葉県千葉市
  • Year and Date: 2014-06-06 – 2014-06-07
• [Presentation] Specific miRNA expressions under chronic starvation stress in mouse hippocampus (2013)
  • Author(s): Shu Takakura
  • Organizer: 第22回世界心身医学会
  • Place of Presentation: リスボン、ポルトガル
• [Presentation] Specific miRNA expressions under chronic starvation stress in mouse hippocampus (2013)
  • Author(s): Shu Takakura
  • Organizer: 第22回世界心身医学会
  • Place of Presentation: リスボン
• [Presentation] Chronic starvation stress alters microRNA expressions in mouse hippocampus (2012)
  • Author(s): Shu Takakura
  • Organizer: 第15回アジア心身医学会
  • Place of Presentation: モンゴル