| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
The hypothalamic paraventricular nucleus (PVN) is one of the regulatory centers for various stress responses. The present study determined whether PPARγ in the PVN is involved in the restraint stress (RS)-induced catecholamine secretion from adrenal medulla in rat. The RS-induced elevation of plasma catecholamines was suppressed by continuous intravenous infusion of 5% glucose. In contrast, pretreatment with intra peritoneal injection with GW9962, an antagonist for PPARγ, abolished the suppression effect. Western blot analyses showed the increase of levels of PPARγ protein in the hypothalamus. Further more, by immunohistochemical analyses revealed that RS gradually increased the expression of PPARγ in the dorsal part and ventral part of the PVN, where preautonomic neurons exist. These results suggest that PPARγ in the preautonomic neurons in the PVN may be involved in RS-induced elevation of plasma catecholamines in rats.
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