| Project/Area Number |
24590909
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General internal medicine (including Psychosomatic medicine)
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| Research Institution | National Hospital Organization, Hizen Psychiatric Center |
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Principal Investigator |
TAKASHIMA YUKI 独立行政法人国立病院機構肥前精神医療センター(臨床研究部), その他部局等, 研究員 (70576404)
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| Co-Investigator(Kenkyū-buntansha) |
YAO Hiroshi 独立行政法人国立病院機構肥前精神医療センター, 臨床研究部, 生化学研究室室長 (20265010)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 大脳白質病変 / プロテオミクス / mass spectrometry / プロテインチップ / 血中タンパク質 / 質量分析 / プロテオミクス解析 / SELDI-TOF-MS / 血中タンパク濃度差軽減法 / 高齢者 / プロテオマイナー / 健診 / SELDI-MS-TOF / ProteoMiner |
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| Outline of Final Research Achievements |
We applied Surface-enhanced laser desorption / ionization - time-of flight mass spectrometry (SELDI -TOF-MS) technology to study proteomic profiles at serum of with white matter lesions in healthy adults. Preliminarily, we did proteo miner protocol and obtained 4 fraction of serum. Then we obtained expression of protein profiles by using strong anion-exchange (Q10) and weak cation-exchange (CM10) and Protein Chip Arrays. With the TOF-MS, mass-to-charge (m/z) or molecular weight (M.W.) of the peptide was determined. The protein expression patterns were analyzed using Biomarker Wizard software, which generates consistent peak intensities were shown as the ratio of intensity values. Result: We went five times of experiments. However, we were not able to find the significantly different peak intensity between two groups. Conclusion: There were no specific protein profiles difference in human serum with or without cerebral white matter lesions.
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