| Project/Area Number |
24590919
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
AKADA Junko 山口大学, 医学部, 特別医学研究員 (30346548)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIKAWA Jun 山口大学, 医学部附属病院, 准教授 (00379950)
OKUDA Masumi 兵庫医科大学, 医学部, 教授 (40531091)
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| Co-Investigator(Renkei-kenkyūsha) |
HIRAYAMA Toshiya 長崎大学, 熱帯医学研究所, 教授 (50050696)
NAKAMURA Kazuyuki 山口大学, 名誉教授 (90107748)
NAKAMURA Mikiko 山口大学, 大学研究推進機構, 学術研究員 (20457310)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | Helicobacter pylori / 血清 / 抗H. pylori抗体 / 抗原タンパク質 / CagA / 小児 / 血清抗体 / H. pylori / プロテオミクス / 抗原解析 / ヘリコバクター・ピロリ / 抗体 |
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| Outline of Final Research Achievements |
This study focused on Japanese child serum antibodies against Helicobacter pylori, a chronically-infected gastric bacterium which causes gastric cancer in adults. Serum samples from 24 children, 22 H. pylori (Hp)-positive and 2 Hp-negative children, were used to catalogue antigenic proteins of a Japanese strain CPY2052 by two-dimensional electrophoresis followed by immunoblot and LC-MS/MS analysis. In total, 24 proteins were identified as candidate antigen proteins. Among these, the major virulence factor, cytotoxin-associated gene A (CagA) protein was the most reactive antigen recognized by all the Hp-positive sera even from children under the age of 3 years. The major antigenic part of CagA was identified in the middle region, and two peptides containing CagA epitopes were identified using a newly developed peptide/protein-combined array chip method. Each of the epitopes was found to contain amino acid residue(s) unique to East Asian CagA.
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