Surrogate marker for sleep disturbances in gastrointestinal diseases

• Project/Area Number: 24590925
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Osaka City University
• Principal Investigator: FUJIWARA YASUHIRO 大阪市立大学, 医学(系)研究科(研究院), 准教授 (40285292)
• Co-Investigator(Kenkyū-buntansha): TOMINAGA Kazunari 大阪市立大学, 大学院医学研究科, 准教授 (80336768) ; WATANABE Toshio 大阪市立大学, 大学院医学研究科, 准教授 (50336773) ; TANIGAWA Tetsuya 大阪市立大学, 大学院医学研究科, 講師 (70423879)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 睡眠障害 / 逆流性食道炎 / マイクロRNA / 胃食道逆流症 / miRNA

Outline of Final Research Achievements

Sleep condition in rats was assessed by electroencephalography using a chronic acid reflux esophagitis model. Wake periods significantly increased in rats with reflux esophagitis compared with control while NREM periods significantly decreased. An increase in short NREM bouts and a decrease in long NREM bouts as well as a decrease in delta power density in rats with reflux esophagitis suggested the presence of sleep fragmentation and poor sleep quality in rats with reflux esophagitis. Such sleep disturbances were improved by the treatment with proton pump inhibitors. Microarray analysis using miRNA in serum exosome showed increased expression of six miRNAs and decreased expression of five miRNAs in rats with reflux esophagitis compared with control.

Research Products

• [Journal Article] Characteristics of nighttime reflux assessed using multichannel intraluminal impedance pH monitoring and a portable electroencephalograph. (2015)
  • Author(s): Fujiwara Y, Kohata Y, Nakahara K, Tanigawa T, Yamagami H, Shiba M, Watanabe K, Tominaga K, Watanabe T, Arakawa T.
  • Journal Title: Dis Esophagus. Volume: 印刷中 Issue: 3 Pages: 278-284
  • DOI: 10.1111/dote.12324
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effects of Esomeprazole on Sleep in Patients with Gastroesophageal Reflux Disease as Assessed on Actigraphy (2015)
  • Author(s): Hiramoto K, Fujiwara Y, Ochi M, Okuyama M, Tanigawa T, Yamagami H, Shiba M, Watanabe K, Watanabe T, Tominaga K, Arakawa T.
  • Journal Title: Internal Medicine Volume: 54 Issue: 6 Pages: 559-565
  • DOI: 10.2169/internalmedicine.54.3718
  • NAID: 130004903059
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Usefulness of baseline impedance in patients with proton pump inhibitor-refractory nonerosive reflux disease. (2015)
  • Author(s): Kohata Y, Fujiwara Y, Yamagami H, Tanigawa T, Shiba M, Watanabe K, Watanabe T, Tominaga K, Arakawa T.
  • Journal Title: J Gastroenterol Hepatol. Volume: 30 Issue: S1 Pages: 36-40
  • DOI: 10.1111/jgh.12743
  • Peer Reviewed / Open Access
• [Journal Article] Acid reflux directly causes sleep disturbances in rat with chronic esophagitis. (2014)
  • Author(s): Nakahara K, Fujiwara Y, Tsukahara T, Yamagami H, Tanigawa T, Shiba M, Tominaga K, Watanabe T, Urade Y, Arakawa T.
  • Journal Title: PLoS One Volume: 9 Issue: 9 Pages: e106969-e106969
  • DOI: 10.1371/journal.pone.0106969
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Overlap in patients with dyspepsia/functional dyspepsia. (2014)
  • Author(s): Fujiwara Y, Arakawa T.
  • Journal Title: J Neurogastroenterol Motil. Volume: 20 Issue: 4 Pages: 447-57
  • DOI: 10.5056/jnm14080
  • Peer Reviewed / Open Access
• [Journal Article] Sleep disturbances and refractory gastroesophageal reflux disease symptoms in patients receiving once-daily proton pump inhibitors and efficacy of twice-daily rabeprazole treatment. (2013)
  • Author(s): Fujiwara Y, Habu Y, Ashida K, Kusano M, Higuchi K, Arakawa T.
  • Journal Title: Digestion. Volume: 88 Issue: 3 Pages: 145-152
  • DOI: 10.1159/000354071
  • Peer Reviewed
• [Journal Article] Gastroesophageal reflux disease and sleep. (2013)
  • Author(s): Fujiwara Y, Arakawa T, Fass R.
  • Journal Title: Gastroenterol Clin North Am. Volume: 42 Issue: 1 Pages: 57-70
  • DOI: 10.1016/j.gtc.2012.11.011
  • Peer Reviewed
• [Journal Article] The association between nighttime reflux and sleep (2013)
  • Author(s): 藤原靖弘平本慶子朴成華 中原憲一木幡幸恵谷川徹也
  • Journal Title: Nippon Shokakibyo Gakkai Zasshi Volume: 110 Issue: 6 Pages: 965-970
  • DOI: 10.11405/nisshoshi.110.965
  • NAID: 10031177023
  • ISSN: 0446-6586, 1349-7693
  • Peer Reviewed
• [Journal Article] Gastroesophageal reflux disease and sleep disturbances. (2012)
  • Author(s): Fujiwara Y, Arakawa T, Fass R.
  • Journal Title: Journal of Gastroenterology Volume: 47 Issue: 7 Pages: 760-769
  • DOI: 10.1007/s00535-012-0601-4
  • NAID: 10030962055
  • Peer Reviewed
• [Journal Article] A multicenter study on the prevalence of Eosinophilic Esophagitis and PPI-responsive esophageal eosinophilic infiltration. (2012)
  • Author(s): Fujiwara Y, Sugawa T, Tanaka F, Tatsuwaki H, Okuyama M, Hayakawa T, Yamamori K, Wada R, Ohtani K, Uno H, Tanigawa T, Watanabe Y, Tominaga K, Watanabe T, et al.
  • Journal Title: Intern Med. Volume: 51 Pages: 3235-9
  • NAID: 130003308153
  • Peer Reviewed
• [Presentation] ラット逆流性食道炎における睡眠障害についての検討 (2014)
  • Author(s): 中原憲一、藤原靖弘、十川光栄、山上博一、谷川徹也、斯波将次、渡辺憲治、冨永和作、渡辺俊雄、荒川哲男
  • Organizer: 第５６回日本消化器病学会大会
  • Place of Presentation: 神戸国際会議場（神戸市、兵庫県）
  • Year and Date: 2014-10-23 – 2014-10-26
• [Presentation] Acid Reflux Directly Causes Sleep Disturbances in Rat Chronic Esophagitis (2014)
  • Author(s): Kenichi Nakahara, Yasuhiro Fujiwara, Tetsuya Tanigawa, Hirokazu Yamagami, Masatsugu Shiba, Kenji Watanabe,
  • Organizer: DDW2014
  • Place of Presentation: Chicago (USA)
  • Year and Date: 2014-05-02 – 2014-05-07
• [Presentation] Changes in Esophageal Microbiome During the Development of Barrett's Esophagus in a Rat Model (2012)
  • Author(s): Kohata Y, Fujiwara Y, Machida H, Okazaki H, Yamagami H, Watanabe K, Watanabe T, Tominaga K, Arakawa T.
  • Organizer: AGA
  • Place of Presentation: サンディエゴコンベンションセンタ-、 アメリカ合衆国
• [Book] 今日の治療指針2015 (2015)
  • Author(s): 藤原靖弘、荒川哲男
  • Total Pages: 2625
  • Publisher: 医学書院
• [Book] 好酸球性消化管疾患診療ガイド (2014)
  • Author(s): 藤原靖弘
  • Total Pages: 103
  • Publisher: 南江堂
• [Remarks] 大阪市立大学大学院医学研究科消化器内科学
  • URL: http://osaka-cu-gastro.jp/gastroenterology/