The expresssion of microRNA in the intestinal metaplasia and gastric carcinoma

• Project/Area Number: 24590926
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Jichi Medical University
• Principal Investigator: Mutoh Hiroyuki 自治医科大学, 医学部, 教授 (50322392)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: microRNA / intestinal metaplasia / Cdx2

Outline of Final Research Achievements

Cdx2-transgenic mouse is the model mouse of the intestinal metaplasia related to gastric carcinoma. The expressions of microRNA in the intestinal metaplasia of Cdx2-transgenic mouse stomach were analyzed. microRNA-21, microRNA-137, and microRNA-145 were elevated in the intestinal metaplasia and gastric carcinoma of Cdx2-transgenic mouse stomach. microRNA-21 downregulated the expression of PTEN, microRNA-137 downregulated the expression of Musashi1. microRNA-145 downregulated the expression of Oct3/4, Sox2, and KLF4.

Research Products

• [Journal Article] Comparative analysis of gastrointestinal microbiota between normal and caudal-related homeobox 2 (cdx2) transgenic mice. (2015)
  • Author(s): Sakamoto H, Asahara T, Chonan O, Yuki N, Mutoh H, Hayashi S, Yamamoto H, Sugano K.
  • Journal Title: Intest Res. Volume: 13 Issue: 1 Pages: 39-34
  • DOI: 10.5217/ir.2015.13.1.39
  • Peer Reviewed / Open Access
• [Journal Article] Cyclooxygenase 2 in gastric carcinoma is expressed in doublecortin- and CaM kinase-like-1-positive tuft cells. (2014)
  • Author(s): Mutoh H, Sashikawa M, Sakamoto H, Tateno T.
  • Journal Title: Gut Liver Volume: 8 Issue: 5 Pages: 508-518
  • DOI: 10.5009/gnl13237
  • Peer Reviewed / Open Access
• [Journal Article] Cyclooxygenase 2 in gastric carcinoma is expressed in doublecortin and CaM kinase-like-1-positive tuft cells. (2014)
  • Author(s): Mutoh H, Sashikawa M, Sakamoto H, Tateno T
  • Journal Title: Gut Liver Volume: in prerss
  • Peer Reviewed
• [Journal Article] SOX9 Is Highly Expressed in Nonampullary Duodenal Adenoma and Adenocarcinoma in Humans. (2013)
  • Author(s): Sakamoto H, Mutoh H, Miura Y, Sashikawa M, Yamamoto H, Sugano K
  • Journal Title: Gut and Liver Volume: 7 Pages: 513-518
  • Peer Reviewed
• [Journal Article] CDX1 directs gastric intestinal metaplasia via induction of stemness-associated reprogramming factors SALL4 and KLF5 (2012)
  • Author(s): Fujii, Y.
  • Journal Title: Proc. Natl. Acad. Sci.USA Volume: 109 Issue: 50 Pages: 20584-20589
  • DOI: 10.1073/pnas.1208651109
  • Peer Reviewed