Analysis of molecular mechanisms and ips genes in mesenchymal stem cells of the patients with advanced gastric cancer.

• Project/Area Number: 24590928
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Nippon Medical School
• Principal Investigator: FUTAGAMI Seiji 日本医科大学, 医学部, 准教授 (50247011)
• Co-Investigator(Kenkyū-buntansha): SAKAMOTO Choitsu 日本医科大学, 大学院医学研究科, 教授 (30196092)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 胃癌幹細胞 / CD 133陽性細胞 / CD 44陽性細胞 / 腹膜播種 / CD133陽性細胞 / CD44陽性細胞 / 癌幹細胞 / CD44 / CD133 / 胃癌 / ips gene / 間葉系幹細胞 / ホーミング

Outline of Final Research Achievements

In our data, poorly differentiated gastric cancer was significantly associated with CD44 expression. In addition. CD44-positive and CD133-positive cells co localized in some gastric cancer tissues. Certain proportion of CD133-positive cells could be seen in as cites of the patients with advanced gastric cancer.

Research Products

• [Journal Article] Leu72Met408 polymorphism of the ghrelin gene is associated with early phase of gastric emptying in the patients with functional dyspepsia in Japan (2015)
  • Author(s): Yamawaki H, Futagami S, Shimpuku M, Shindo T, Maruki Y, et al
  • Journal Title: J Neurogastroenterol Motil Volume: 21 Issue: 1 Pages: 93-102
  • DOI: 10.5056/jnm14086
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Systematic review with meta-analysis:post-infectious functional dyspepsia (2015)
  • Author(s): Futagami S, Itoh T, Sakamoto C.
  • Journal Title: Aliment Pharmacol Ther Volume: 41 Issue: 2 Pages: 177-188
  • DOI: 10.1111/apt.13006
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Apurinic/apyrimidinic endonuclease-1 is associated with angiogenesis and VEGF production via upregulation of COX-2 expression in esophageal cancer tissues. (2014)
  • Author(s): Nagoya H, Futagami S, Shimpuku M, et al.
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: 306
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Impact of sleep disorders, quality of life and gastric emptying in distinct subtypes of functional dyspepsia in Japan (2014)
  • Author(s): Yamawaki H, Futagami S, Shimpuku M, et al.
  • Journal Title: J Neurogastroenterol Motil Volume: 20 Pages: 104-112
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Impact of eating attitude and impairment of physical quality of life between tertiary clinic and primary clinic functional dyspepsia outpatients in Japan (2014)
  • Author(s): Shimpuku M, Futagami S, Tajima N, et al.
  • Journal Title: J Neurogastroenterol Motil Volume: 20 Issue: 4 Pages: 506-515
  • DOI: 10.5056/jnm14015
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Apurinic/apyrimidinic endonuclease-1 is associated with angiogenesis and VEGF production via upregulation of COX-2 expression in esophageal cancer tissues. (2014)
  • Author(s): Nagoya H, Futagami S, Shimpuku M, Tatsuguchi A, Wakabayashi T, Yamawaki H, Kodaka Y, Kawagoe T, Watarai Y, Makino H, Miyashita M, Tsuchiya S, Crowe SE, Sakamoto C.
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: 306 Issue: 3 Pages: G183-G190
  • DOI: 10.1152/ajpgi.00057.2013
  • Peer Reviewed
• [Journal Article] Impact of sleep disorders, quality of life and gastric emptying in distinct subtypes of functional dyspepsia in Japan (2014)
  • Author(s): Yamawaki H, Futagami S, Shimpuku M, et al. (計11名)
  • Journal Title: J Neurogastroenterol Motil Volume: Vol. 20 No. 1 Issue: 1 Pages: 104-12
  • DOI: 10.5056/jnm.2014.20.1.104
  • Peer Reviewed
• [Journal Article] Impact of coexisting irritable bowel syndrome and non-erosive reflux disease on postprandial abdominal fullness and sleep disorders in functional dyspepsia. (2013)
  • Author(s): Futagami S, Yamawaki H, Shimpuku M, Izumi N, Wakabayashi T, Kodaka Y, Nagoya H, Shindo T, Kawagoe T, Sakamoto C.
  • Journal Title: J Nippon Med Sch Volume: 80 Pages: 362-370
  • NAID: 130004147250
  • Peer Reviewed
• [Journal Article] Water-soluble vitamin deficiencies in complicated peptic ulcer patients soon after ulcer onset in Japan. (2013)
  • Author(s): Miyake K, Akimoto T, Kusakabe M, Sato W, Yamada A, Yamawaki H, Kodaka Y, Shimpuku M, Nagoya H, Shindo T, Ueki N, Kusunoki M, Kawagoe T, Futagami S, Tsukui T, Sakamoto C.
  • Journal Title: J Nutr Sci Vitaminol Volume: 59 Pages: 503-508
  • NAID: 130003393746
  • Peer Reviewed
• [Journal Article] Sleep disorders in functional dyspepsia and future therapy. (2013)
  • Author(s): Futagami S, Shimpuku M, Yamawaki H, Izumi N, Kodaka Y, Nagoya H, Wakabayashi T, Shindo T, Kawagoe T, Sakamoto C
  • Journal Title: J Nippon Med Sch Volume: 80 Pages: 104-109
  • NAID: 130004147211
  • Peer Reviewed
• [Journal Article] Impact of sleep disorders in Japanese patients with functional dyspepsia (FD):nizatidine improves clinical symptoms, gastric emptying and sleep disorders in FD patients. (2013)
  • Author(s): Futagami S, Yamawaki H, Izumi N, Shimpuku M, Kodaka Y, Wakabayashi T, Nagoya H, Shindo T, Kawagoe T, Gudis K, Itoh T, Sakamoto C.
  • Journal Title: J Gastroenterol Heapatol Volume: 53 Issue: 8 Pages: 53-60
  • DOI: 10.1111/jgh.12236
  • Peer Reviewed
• [Journal Article] Apurinic/apyrimidinic endonuclease-1 (APE-1) is overexpressed via the activation of NF-κB-p65 in MCP-1-positive esophageal squamous cell carcinoma tissue (2013)
  • Author(s): Song J, Futagami S, Nagoya H, Kawagoe T, Yamawaki H, Kodaka Y, et al.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 52 Issue: 2 Pages: 112-119
  • DOI: 10.3164/jcbn.12-95
  • NAID: 130004466710
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] The preproghrelin 3056TT genotype is associated with the feeling of hunger and low acylated ghrelin levels in Japanese patients with H. pylori-negative functional dyspepsia. (2013)
  • Author(s): Futagami S, Shimpuku M, Kawagoe T, Izumi N, et al.
  • Journal Title: Internal Medicine Volume: 52 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Nizatidine improves clinical symptoms and gastric emptying in patients with functional dyspepsia accompanied by impaired gastric emptying. (2012)
  • Author(s): Futagami S, Shimpuku M, Song JM, Kodaka Y, Yamawaki H, Nagoya H, Shindo T, Kawagoe T, Gudis K, Horie A, Gudis K, Iwakiri K, Sakamoto C.
  • Journal Title: Digestion Volume: 86 Pages: 114-121
  • Peer Reviewed
• [Journal Article] Classification of patients who experience a higher distress level to transoral esophagogastroduodenoscopy than to transnasal esophagogastroduodenoscopy. (2012)
  • Author(s): Miyake K, Kusunoki M, Ueki N, Yamada A, Nagoya H, Kodaka Y, Shindo T, Kawagoe T, Gudis K, Futagami S, Tsukui T, Sakamoto C.
  • Journal Title: Dig Endosc Volume: 53 Issue: 4 Pages: 66-73
  • DOI: 10.1111/den.12006
  • Peer Reviewed
• [Presentation] EPS-PDS overlap群における睡眠障害、QOL、胃排出能の比較検討の試み (2014)
  • Author(s): 2.山脇博士、二神生爾、新福摩弓、坂本長逸
  • Organizer: 第46回胃病態機能研究会
  • Place of Presentation: 横浜
  • Year and Date: 2014-08-12 – 2014-08-13
• [Presentation] Apurinic/apyrimidinic endonucleas-1 (APE-1)によるSTAT 3を介した食道癌進展機構の解析 (2014)
  • Author(s): 3.名児耶浩幸、二神生爾、坂本長逸
  • Organizer: 第100回 日本消化器病学会
  • Place of Presentation: 東京
  • Year and Date: 2014-04-23 – 2014-04-26
• [Presentation] H. pylori陽性胃炎患者におけるCOX-2のSNPを用いた除菌後ハイリスク群の絞込み (2014)
  • Author(s): 小高康裕、二神生爾、坂本長逸
  • Organizer: 第100回 日本消化器病学会
  • Place of Presentation: 東京
  • Year and Date: 2014-04-23 – 2014-04-26
• [Presentation] LPS前投与urocortin 1脳槽内投与ラットにおける胃排出能および小腸通過時間の比較検討 (2013)
  • Author(s): 山脇博士，二神生爾，他.
  • Organizer: 第50回日本臨床生理学会
  • Place of Presentation: 東京
• [Presentation] FD患者におけるGN-βおよびghrelin の遺伝子多型と胃排出能との相関関係 (2013)
  • Author(s): 新福摩弓，二神生爾、他
  • Organizer: 第50回日本臨床生理学会
  • Place of Presentation: 東京
• [Presentation] 食道癌組織においてROS刺激下に誘導されるApurinic/apyrimidinic endonuclease-1(APE-1)およびCOX-2発現意義の解析 (2013)
  • Author(s): 名児耶浩幸、二神生爾、坂本長逸
  • Organizer: 日本消化管学会
  • Place of Presentation: 東京
• [Presentation] 機能性ディスペプシア患者における空腹感スコアとGHRL遺伝子多型との相関関係の検討 (2013)
  • Author(s): 二神生爾，山脇博士，新福摩弓，他
  • Organizer: 日本消化器病学会
  • Place of Presentation: 鹿児島
• [Presentation] CD44陽性胃癌幹細胞のCOX-2発現の解析 (2012)
  • Author(s): 山脇博士，二神生爾，河越哲郎，小高康裕，名児耶浩幸，他
  • Organizer: 日本ヘリコバクター学会
  • Place of Presentation: 岡山
• [Presentation] COX-2のSNPを用いたH. pylori胃炎患者のハイリスク群の絞り込みの検討 (2012)
  • Author(s): 小高康裕、二神生爾、山脇博士、他
  • Organizer: 日本消化器病学会
  • Place of Presentation: 神戸
• [Presentation] ROS刺激下流の新規分子マーカー，apurinic/apyrimidinic endonuclease-1 (APE-1)を用いた食道癌進展および化学療法感受性の解析
  • Author(s): 名児耶浩幸，二神生爾，他
  • Organizer: 第99回日本消化器病学会 大会
  • Place of Presentation: 鹿児島