Functional analysis of small GTP binding protein Ral in colitis-associated cancer
| Project/Area Number |
24590941
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kyoto University |
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Principal Investigator |
NAKASE Hiroshi 京都大学, 医学(系)研究科(研究院), 講師 (60362498)
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| Research Collaborator |
HONZAWA Yusuke
MATSUMURA Kayoko
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 炎症性腸疾患 / 炎症性大腸発がん / Ral / 自然免疫 / 炎症性大腸がん / サイトカイン / 炎症性大腸発癌 / IL-17 / IL-1beta |
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| Outline of Final Research Achievements |
The aim of this study is to elucidate the role of Small GTP binding protein Ral in development of colitis-associated cancer (CAC). We used Ral-GAPα2 KO mice (Ral continuously activated) for this study. The gene expression of pro-inflammatory cytokines was significantly up-regulated in colonic mucosa of Ral-GAPα2 KO mice than in wild type mice. Interestingly, Ral activation accelerated colonic inflammation of IL-10KO mice. In addition, we made CAC model by the injection of azoxymethane (AOM) with repeated administration of dextran sulfate sodium (DSS). The number of CAC with submucosal invasion was higher in Ral-GAPα2 KO mice treated with AOM and DSS than in wild type mice. These data indicate that Ral is involved in innate immune system and providing invasive phenotype in CAC.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Use of capsule endoscopy in patients with Crohn's disease in Japan: a multicenter survey.2014
Author(s)
Esaki M, Matsumoto T, Watanabe K, Arakawa T, Naito Y, Matsuura M, Nakase H, Hibi T, Matsumoto T, Nouda S, Higuchi K, Ohmiya N, Goto H, Kurokawa S, Motoya S, Watanabe M.
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Journal Title
J Gastroenterol Hepatol
Volume: 29(1)
Issue: 1
Pages: 96-101
DOI
Related Report
Peer Reviewed
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[Journal Article] Involvement of interleukin-17A-induced expression of heat shock protein 47 in intestinal fibrosis in Crohn's disease2014
Author(s)
Honzawa Y, Nakase H, Shiokawa M, Yoshino T, Imaeda H, Matsuura M, Kodama Y, Ikeuchi H, Andoh A, Sakai Y, Nagata K, Chiba T
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Journal Title
Gut
Volume: 63
Issue: 12
Pages: 1902-12
DOI
Related Report
Peer Reviewed
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[Journal Article] Therapeutic effect of intensve granulocyte and monocyte adsorption apheresis combined with thiopurines for steroids and biologics naive Japanese patients with early-diagnosed Crohn's Disease2014
Author(s)
Fukuchi T, Nakase H, Ubukata S, Matsuura M, Yoshino T, Toyonaga T, Koga H, Eguchi T, Yamashita H, Ito D, Ashida K.
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Journal Title
BMC Gastroenterol
Volume: 13
Issue: 1
Pages: 124-124
DOI
Related Report
Peer Reviewed
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