| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The aim of this study is to elucidate the role of Small GTP binding protein Ral in development of colitis-associated cancer (CAC). We used Ral-GAPα2 KO mice (Ral continuously activated) for this study. The gene expression of pro-inflammatory cytokines was significantly up-regulated in colonic mucosa of Ral-GAPα2 KO mice than in wild type mice. Interestingly, Ral activation accelerated colonic inflammation of IL-10KO mice. In addition, we made CAC model by the injection of azoxymethane (AOM) with repeated administration of dextran sulfate sodium (DSS). The number of CAC with submucosal invasion was higher in Ral-GAPα2 KO mice treated with AOM and DSS than in wild type mice. These data indicate that Ral is involved in innate immune system and providing invasive phenotype in CAC.
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